M.S. / Biochemistry / Culture parameters influencing metabolism of synthetic [superscript 14] C-labeled lignin model compounds to [superscript 14] CO2 in defined media by the fungi, Polyporus versicolor and Phanerochaete chrysosporium, were examined. Model compound metabolism was oxygen-dependent. Agitation of the cultures, resulting in formation of mycelial pellets, suppressed [superscript 14] CO2 evolution by P. chrysosporium, to a greater extent than by-P. versicolor. The concentration of nutrient nitrogen was critical; [superscript 14] CO2 evolution was retarded at 12 mM ammonium tartrate relative to 1.2 mM ammonium tartrate. Cultures evolved more [superscript 14] CO2 when grown on xylose than on either glucose or glycerol. Initial glucose at 0.1%concentration was significantly less supportive of growth and [superscript 14] CO2 evolution than cultures with 0.5-1.0% glucose. Studies with cycloheximide, a protein synthesis inhibitor, demonstrated that the lignin model compound degrading enzyme system was constitutive. 4-Methoxyl-[ [superscript 14]C] veratryl alcohol was found to be a catabolic product in the metabolism of 4-methoxyl-[ [superscript 14]C]veratrylglycerol-β-guaiacyl ether and 4-methoxyl-[ [superscript 14]C] veratric acid to [superscript 14]CO2.
| Identifer | oai:union.ndltd.org:OREGON/oai:content.ohsu.edu:etd/133 |
| Date | 01 1900 |
| Creators | Weinstein, David Allen |
| Publisher | Oregon Health & Science University |
| Source Sets | Oregon Health and Science Univ. Library |
| Language | English |
| Detected Language | English |
| Type | Text |
| Format | Needs Adobe Acrobat Reader to view., pdf, 1362.254 KB |
| Rights | http://www.ohsu.edu/library/etd_rights.shtml |
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