by Chan Wing-yee. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1993. / Includes bibliographical references (leaves 93-95). / ACKNOWLEDGEMENT --- p.i / ABSTRACT --- p.ii / Chapter CHAPTER 1 --- INTRODUCTION --- p.1 / Chapter 1.1 --- General Introduction --- p.1 / Chapter 1.2 --- "Clinical Significance of Fe, Cu and Zn" --- p.2 / Chapter 1.3 --- Alternative Methods of Analysis --- p.5 / Chapter 1.4 --- Principles of Energy Dispersive X-ray Fluorescence Spectrometry --- p.11 / Chapter 1.5 --- Research Plan --- p.20 / Chapter CHAPTER 2 --- EXPERIMENTAL --- p.22 / Chapter 2.1 --- Energy Dispersive X-ray Fluorescence Analysis --- p.22 / Chapter 2.1.1 --- Apparatus --- p.22 / Chapter 2.1.2 --- Reagents --- p.25 / Chapter 2.1.3 --- Procedure --- p.28 / Chapter 2.2 --- Atomic Absorption Spectrometric Analysis --- p.32 / Chapter 2.2.1 --- Apparatus --- p.32 / Chapter 2.2.2 --- Reagents --- p.32 / Chapter 2.2.3 --- Procedure --- p.34 / Chapter CHAPTER 3 --- RESULTS AND DISCUSSION --- p.38 / Chapter 3.1 --- Optimisation of Excitation Conditions --- p.38 / Chapter 3.1.1 --- Effect of Filter --- p.38 / Chapter 3.1.2 --- Effect of Tube Voltage --- p.43 / Chapter 3.1.3 --- Effect of Tube Current --- p.44 / Chapter 3.2 --- Optimisation of Preconcentration Procedure --- p.46 / Chapter 3.2.1 --- Effect of Sample Area and Collimator Size --- p.46 / Chapter 3.2.2 --- Effect of pH --- p.51 / Chapter 3.2.3 --- Effect of Ligand Concentration --- p.54 / Chapter 3.2.4 --- Effect of Mixing Time --- p.57 / Chapter 3.2.5 --- Effect of Standing Time --- p.59 / Chapter 3.2.6 --- Study of Sample Homogeneity --- p.61 / Chapter 3.3 --- Optimisation for Deproteination --- p.63 / Chapter 3.3.1 --- Effect of Different Protein Precipitants --- p.63 / Chapter 3.3.2 --- Effect of Trichloroacetic Acid Concentration --- p.65 / Chapter 3.3.3 --- Effect of Hydrochloric Acid Concentration --- p.67 / Chapter 3.3.4 --- Effect of Temperature --- p.69 / Chapter 3.3.5 --- Effect of Incubation Time --- p.71 / Chapter 3.4 --- Study of Blanks --- p.74 / Chapter 3.5 --- Construction of Calibration Curves --- p.77 / Chapter 3.6 --- Determination of Detection Limit and Sensitivity --- p.84 / Chapter 3.7 --- Accuracy and Reproducibility Tests --- p.86 / Chapter 3.8 --- Parallel Check --- p.89 / Chapter CHAPTER 4 --- CONCLUSION --- p.92 / REFERENCES --- p.93
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_319226 |
| Date | January 1993 |
| Contributors | Chan, Wing-yee., Chinese University of Hong Kong Graduate School. Division of Chemistry. |
| Publisher | Chinese University of Hong Kong |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, iii, 95 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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