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Use of the yeast two-hybrid system to define the function of THAP5 protein

THAP5 is a protein which was recently isolated in the Zervos Lab as an interactor of a pro-apoptotic protein, Omi/HtrA2. THAP5 is unique because it shares no homology with mouse or rat and can only be found in humans. The only homology it shares with any other protein is its THAP domain. THAP proteins are zinc-dependent sequence specific DNA-binding factors belonging to the zinc-finger family of proteins (2). There are 12 identified members of TIIAP proteins in humans, THAP0-THAP 11. The roles of these THAP proteins include proliferation, apoptosis, cell cycle, chromosome segregation, chromosome modification, and transcriptional regulation (2). The function of THAP5 is still unclear and thus, a Yeast Two-Hybrid experiment will be done to further determine its function. The Yeast Two-Hybrid System is a common molecular biology technique used to identify interactors of a certain protein of interest. By identifying the protein interactors of THAP5 and their functions, it is possible to further determine the function of THAP5.

Identiferoai:union.ndltd.org:ucf.edu/oai:stars.library.ucf.edu:honorstheses1990-2015-1849
Date01 January 2009
CreatorsPopat, Paiyal V.
PublisherSTARS
Source SetsUniversity of Central Florida
LanguageEnglish
Detected LanguageEnglish
Typetext
SourceHIM 1990-2015

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