Chan Lai-Yin. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (leaves 83-84). / Abstracts in English and Chinese. / Abstract --- p.i / Acknowledgements --- p.iii / Table of Contents --- p.iv / List of Abbreviations and symbols --- p.viii / List of Appendices --- p.x / List of Figures --- p.xi / List of Tables --- p.xii / Chapter Chapter 1: --- General Introduction --- p.1 / Chapter 1.1 --- Studies of Plant Constituents --- p.1 / Chapter 1.2 --- Extraction of Chemical Ingredients --- p.2 / Chapter 1.3 --- Separation and Purification of Chemical Ingredients --- p.2 / Chapter 1.3.1 --- Thin Layer Chromatography (TLC) --- p.3 / Chapter 1.3.2 --- Open Column Chromatography (OCC) --- p.4 / Chapter 1.4 --- Methods of Structural Identification --- p.4 / Chapter 1.4.1 --- Nuclear Magnetic Resonance (NMR) --- p.5 / Chapter 1.4.1.1 --- Proton NMR Spectroscopy (1H-NMR) --- p.5 / Chapter 1.4.1.2 --- Carbon-13 NMR Spectroscopy (13C NMR) --- p.5 / Chapter 1.4.1.3 --- Distortionless Enhancement by Polarization Transfer (DEPT) Experiment --- p.6 / Chapter 1.4.1.4 --- Two-dimensional Homonuclear Correlation Experiment --- p.6 / Chapter 1.4.1.5 --- Two-dimensional Heternuclear Chemical shift Correlation Experiment --- p.7 / Chapter 1.4.2 --- Mass Spectroscopy --- p.7 / Chapter 1.5 --- Literature Review --- p.8 / Chapter 1.5.1 --- Medical information on the genus Zanthoxylum --- p.8 / Chapter 1.5.2 --- Compounds found in the genus Zanthoxylum --- p.8 / Chapter 1.5.3 --- Compounds found in the Zanthoxylum acanthopodium --- p.9 / Chapter 1.5.4 --- Biological activities of the genus Zanthoxylum --- p.9 / Chapter 1.6 --- Research plan and Objectives of the present study --- p.16 / Chapter 1.7 --- References --- p.17 / Chapter Chapter 2: --- Materials and Methods --- p.20 / Chapter 2.1 --- General experimental procedures --- p.20 / Chapter 2.1.1 --- Solvents --- p.20 / Chapter 2.1.2 --- Chromatographic methods --- p.20 / Chapter 2.1.2.1 --- Normal phase chromatography --- p.20 / Chapter 2.1.2.2 --- Macro-reticular resinous adsorption chromatography --- p.20 / Chapter 2.1.3.3 --- Normal phase preparative thin layer chromatography --- p.21 / Chapter 2.1.3.4 --- Thin layer chromatography --- p.21 / Chapter 2.1.3 --- Determination of physical data --- p.21 / Chapter 2.1.3.1 --- Melting point determination --- p.21 / Chapter 2.1.3.2 --- Optical Rotation (OR) --- p.21 / Chapter 2.1.3.3 --- Ultraviolet (UV) absorption spectra --- p.22 / Chapter 2.1.3.4 --- Infra-red (IR) absorption spectra --- p.22 / Chapter 2.1.3.5 --- Nuclear Magnetic Resonance spectra (NMR) --- p.22 / Chapter 2.1.3.6 --- Mass Spectra (MS) --- p.22 / Chapter Chapter 3: --- Results / Chapter 3.1 --- "Procurement, extraction and initial fractionation of dried leaves and stem bark of Zahthoxylum acanthopodium" --- p.23 / Chapter 3.2 --- Chromatographic separation of the hexane and ethyl acetate extract --- p.24 / Chapter 3.2.1 --- Column chromatographic separation of fraction HEA-E --- p.24 / Chapter 3.2.1.1 --- Characterization of β -sitosterol --- p.24 / Chapter 3.2.2 --- Column chromatographic separation of fraction HEA-F --- p.25 / Chapter 3.2.2.1 --- Characterization of β -amyrin --- p.25 / Chapter 3.2.2.2 --- Characterization of 1 - octacosanol --- p.26 / Chapter 3.2.2.3 --- Characterization of (-)-sesamin --- p.26 / Chapter 3.2.3 --- Column chromatographic separation of fraction HEA-G --- p.27 / Chapter 3.2.3.1 --- Characterization of methyl hexacosanoate --- p.27 / Chapter 3.2.3.2 --- Characterization of methyl pluviatilol --- p.28 / Chapter 3.2.4 --- Column chromatographic separation of fraction HEA-H --- p.28 / Chapter 3.2.4.1 --- Characterization of (+)-methyl piperitol --- p.29 / Chapter 3.2.4.2 --- Characterization of (-)-epieudesmin --- p.29 / Chapter 3.2.5 --- Column chromatographic separation of fraction HEA-K --- p.29 / Chapter 3.2.5.1 --- Characterization of (+)-syringaresinol --- p.30 / Chapter 3.2.6 --- Column chromatographic separation of fraction HEA-M --- p.30 / Chapter 3.2.6.1 --- Characterization of daucosterol 6'-stearate --- p.31 / Chapter 3.2.7 --- Column chromatographic separation of fraction HEA-P --- p.31 / Chapter 3.2.7.1 --- Characterization of β -sitosterol- β -D-glucoside --- p.31 / Chapter 3.3 --- Chromatographic separation of the n-butanol extract --- p.32 / Chapter 3.3.1 --- Column chromatographic separation of fraction Bu-I --- p.32 / Chapter 3.3.1.1 --- Characterization of collettiside III --- p.32 / Chapter 3.3.1.2 --- Characterization of gracilline --- p.33 / Chapter Chapter 4: --- Discussion --- p.35 / Chapter 4.1 --- Isolated compounds --- p.35 / Chapter 4.1.1 --- Sterols --- p.35 / Chapter 4.1.1.1 --- Identification of -sitosterol --- p.36 / Chapter 4.1.1.2 --- Identification of β -sitosterol- β -D-glucoside --- p.41 / Chapter 4.1.1.3 --- Identification of daucosterol 6'-stearate --- p.45 / Chapter 4.1.2 --- Saponins --- p.50 / Chapter 4.1.2.1 --- Steroid glycosides --- p.51 / Chapter 4.1.2.1.1 --- Identification of collettiside III --- p.51 / Chapter 4.1.2.1.2 --- Identification of gracilline --- p.55 / Chapter 4.1.3 --- Terpenoid --- p.60 / Chapter 4.1.3.1 --- Identification of β -amyrin --- p.61 / Chapter 4.1.4 --- Lignans --- p.66 / Chapter 4.1.4.1 --- Identification of (-)-sesamin --- p.68 / Chapter 4.1.4.2 --- Identification of (-)-epieudesmin --- p.69 / Chapter 4.1.4.3 --- Identification of (+)-methyl piperitol --- p.70 / Chapter 4.1.4.4 --- Identification of methyl pluviatilol --- p.71 / Chapter 4.1.4.5 --- Identification of (+)-syringaresinol --- p.72 / Chapter 4.1.5 --- Alkanol --- p.78 / Chapter 4.1.5.1 --- Identification of 1 -octacosanol --- p.78 / Chapter 4.1.5.2 --- Identification of methyl hexacosanoate --- p.79 / Chapter 4.2 --- Conclusion --- p.81 / Chapter 4.3 --- References --- p.83
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_324281 |
| Date | January 2003 |
| Contributors | Chan, Lai-Yin., Chinese University of Hong Kong Graduate School. Division of Chemistry. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xii, 95 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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