Alpha zearalenol is a derivative of the mycotoxin zearalenone, which is commonly found in agricultural crops and commercial feed. It is a secondary metabolite of filamentous fungi of the genus Fusarium. The alpha zearalenol is the result of a biotransformation of zearalenone in the liver and intestinal tissue. When the zearalenone is metobolised this way, it is able to compete for the binding sites of estrogen receptors because the structure is very similar to 17 beta estradiol.
Toxicity of alpha zearalenol therefore lies in an estrogenic stimulation, which induces morphological and functional changes in the reproductive organs and gametes. Toxicity is manifested on the male gamete by reducing viability, motility and the portion of sperms capable of undergoing acrosome reaction.
The aim of this study was to evaluate the effect of alpha zearalenol on the hyperactivation and acrosome reaction of boar sperm depending on the concentration and duration of exposure to mycotoxins.
For the purposes of this study we used two methodologies and the short term chilled commercial insemination doses which were kept in a box with the temperature of 17 degrees of Celsius. The two methodologies had a different count of used concentrations of alpha zearalenol and exposure time. Acrosome reaction of sperms was assessed in both methodologies, while the share of hyperactive sperm was assessed only in the second methodology.
The first methodology did not prove any effect of alpha zearalenol on acrosome reaction, but the second methodology has proven a positive effect of concentrations 5, 10 and 20 uM alpha zearalenol on acrosome reaction of boar sperm. Individual values of parameters from CASA analysis (VCL, ALH, LIN and WOB), which were used for evaluation of hyperactivation of boar sperm, were not affected by any used concentrations of alpha zearalenol.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:257894 |
| Date | January 2016 |
| Creators | Doleželová, Kateřina |
| Contributors | Rajmon, Radko, Marie, Marie |
| Publisher | Česká zemědělská univerzita v Praze |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
Page generated in 0.0017 seconds