Monocarboxylate transporters (MCTs) have been shown to be important in regulating metabolism during hypoxia in mammals. However, the role of MCTs in hypoxic survival in lower vertebrates is currently unclear. The goal of this study was to investigate the coordination of MCTs along with other metabolic proteins during hypoxia. Therefore, we subjected zebrafish (Danio rerio) to 1.5 mg L-1 O2 over 48 and 96-hr and measured tissue-specific transcriptional changes of MCTs (1, 2 and 4), lactate dehydrogenase A (LDHa), citrate synthase (CS), and other metabolic proteins using real-time RT-PCR. There were no changes in mRNA in muscle at 48 and 96-hr.
When data from both time points were pooled in brain, a significant increase was found in MCT4 (+102%) and LDHa (+28%) mRNA indicating a preference towards glycolysis. In gills, there were increases in LDHa at 48-hr (+101%) and MCT1 (+24%) mRNA from pooled data suggesting that both anaerobic and aerobic metabolism is being utilized. Heart had the greatest changes in transcriptional levels compared to other tissues. At 48-hr, increases were found in MCT1 (+117%), MCT4 (+86%), LDHa (+197%), and pooled data showed an increase in CS (+18%) mRNA. These results indicate that the influx and efflux of lactate are both employed as strategies in cardiac tissue during hypoxia. This study has shown that fish utilize tissue-specific regulation of MCTs along with other metabolic genes during hypoxia. / Thesis (Master, Biology) -- Queen's University, 2009-08-24 13:44:06.114
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:OKQ.1974/5095 |
| Date | 26 August 2009 |
| Creators | NGAN, ADAM K. |
| Contributors | Queen's University (Kingston, Ont.). Theses (Queen's University (Kingston, Ont.)) |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English, English |
| Detected Language | English |
| Type | Thesis |
| Format | 273972 bytes, application/pdf |
| Rights | This publication is made available by the authority of the copyright owner solely for the purpose of private study and research and may not be copied or reproduced except as permitted by the copyright laws without written authority from the copyright owner. |
| Relation | Canadian theses |
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