Submitted by Sara Ribeiro (sara.ribeiro@ucb.br) on 2018-11-22T11:48:56Z
No. of bitstreams: 1
CarolinaRodriguesChanesDissertacao2018.pdf: 2882728 bytes, checksum: 9c589bc58e82d7391f2534b1161bc575 (MD5) / Approved for entry into archive by Sara Ribeiro (sara.ribeiro@ucb.br) on 2018-11-22T11:49:14Z (GMT) No. of bitstreams: 1
CarolinaRodriguesChanesDissertacao2018.pdf: 2882728 bytes, checksum: 9c589bc58e82d7391f2534b1161bc575 (MD5) / Made available in DSpace on 2018-11-22T11:49:14Z (GMT). No. of bitstreams: 1
CarolinaRodriguesChanesDissertacao2018.pdf: 2882728 bytes, checksum: 9c589bc58e82d7391f2534b1161bc575 (MD5)
Previous issue date: 2018-08-24 / Zika virus is an emergent flavivirus transmitted by mosquitos of Aedes genus, was first isolated in 1947 from Zika forest in Africa of the sentinel?s monkeys. The virus spread to other continents and was related with congenital and neurological syndromes in South America and became a public health issue. The genomic sequencing arouses as a crucial tool to obtain answers in real time about infections diseases outbreaks and has helped to understand the origin, phylogeny and trace Zika virus propagation. The main goals of this work are to obtain the complete Zika virus genome sequences that circulated in three different states of Brazil, DF, MT and TO, using the amplification technic multiplex PCR, the MinION portable platform of Oxford Nanopore. Besides that, we had the goal to generate the phylogenetic tree by maximum likelihood to better understand the origin, propagation and dissemination of the virus in these states, and consequentially, in Brazil. The sequences obtain in this present work shown high coverage, 94% to 100% was the coverage of the completeness of the genomes, that presented four distinct lineages, two different clades and related with Northeast samples of Brazil. Finally, our study provides new complete genome sequences of Zika virus in the state of Mato Grosso, demonstrate the relationship of the genome sequences of MT with sequences of Northeast of Brazil and suggests two possible entrances of Zika virus in the state of MT. / Zika virus ? um flaviv?rus emergente transmitido por mosquitos do g?nero Aedes, primeiramente isolado em 1947 de macacos sentinelas na floresta de Zika na ?frica. O v?rus se espalhou para outros continentes at? ser relacionado com s?ndromes neurol?gicas e cong?nitas na Am?rica do Sul sendo taxado como um problema de sa?de p?blica. O sequenciamento gen?mico emergiu como uma ferramenta crucial de respostas em tempo real a surtos de doen?as infecciosas e tem ajudado a entender a filogenia, origem e delinear a propaga??o do Zika virus. Este trabalho tem como objetivos obter a sequ?ncia completa do Zika virus circulante em tr?s diferentes estados do Brasil, DF, MT e TO, utilizando a t?cnica de amplifica??o por PCR multiplex, a plataforma port?til MinION da Oxford Nanopore. Al?m disto, tivemos como objetivo realizar a ?rvore filogen?tica por m?xima verossimilhan?a a fim de compreender melhor a origem, propaga??o e dissemina??o do v?rus nestes estados e, consequentemente, no Brasil. As sequ?ncias obtidas no presente trabalho apresentaram alta cobertura, foi poss?vel cobrir entre 94% e 100% dos genomas que se apresentaram em quatro linhagens distintas e se associaram em dois clados diferentes relacionados as amostras do Nordeste do Brasil. Finalmente, nosso estudo fornece novas sequ?ncias completas do Zika virus no estado do Mato Grosso, demonstra a rela??o das sequencias do MT com as sequ?ncias do Nordeste do Brasil e sugere duas poss?veis entradas do Zika virus no estado do MT.
| Identifer | oai:union.ndltd.org:IBICT/oai:bdtd.ucb.br:tede/2499 |
| Date | 24 August 2018 |
| Creators | Chanes, Carolina Rodrigues |
| Contributors | Costa, Fabr?cio Falconi, Melo, Fernando Lucas |
| Publisher | Universidade Cat?lica de Bras?lia, Programa Stricto Sensu em Ci?ncias Gen?micas e Biotecnologia, UCB, Brasil, Escola de Sa?de e Medicina |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UCB, instname:Universidade Católica de Brasília, instacron:UCB |
| Rights | info:eu-repo/semantics/openAccess |
Page generated in 0.0019 seconds