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Isolation of ABA Insensitive Mutants using a Sensitized Screen

ABA insensitive mutants (abi1 - abi5) have been isolated in germination screens that use high concentrations of exogenous ABA, and this method is believed to be saturated. To overcome this problem, a sensitized screen that used much lower concentration of exogenous ABA was performed to isolate new ABA insensitive mutants. Some of the isolated mutants had defects in light or retrograde signalling. One particular mutant (18-11) developed long hypocotyls under normal light condition. Based on its response to blue, red and far-red light conditions, this mutant is likely a novel hy mutant. Genetic analysis revealed that while ABA insensitivity in this mutant is recessive, the long hypocotyl phenotype is dominant. Positional cloning is currently being carried out to identify the gene. Findings from this study supports that ABA signalling interacts with light signalling networks.

Identiferoai:union.ndltd.org:TORONTO/oai:tspace.library.utoronto.ca:1807/24611
Date27 July 2010
CreatorsNam, Eric Hyung-Uk
ContributorsMcCourt, Peter
Source SetsUniversity of Toronto
Languageen_ca
Detected LanguageEnglish
TypeThesis

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