Kong Pui Shan. / Thesis submitted in: November 2004. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2005. / Includes bibliographical references (leaves 164-185). / Abstracts in English and Chinese. / Table of Contents --- p.i / List of Figures and Tables --- p.iii / List of Abbreviations --- p.viii / Chapter Chapter 1: --- Introduction --- p.1 / Chapter 1.1 --- Endocrine system and the pituitary gland in teleosts --- p.1 / Chapter 1.2 --- The biology of black seabream (Acanthopagrus schlegeli) --- p.4 / Chapter 1.3 --- Seasonal variation of gene expression in black seabream pituitary --- p.7 / Chapter 1.4 --- Expressed sequence tag (EST) analysis --- p.9 / Chapter 1.5 --- Basic Local Alignment Search Tool (BLAST) --- p.11 / Chapter 1.6 --- Aim of this study --- p.14 / Chapter Chapter 2: --- Materials and Methods --- p.15 / Chapter 2.1 --- Preliminary EST analysis of black seabream pituitary cDNA libraries --- p.15 / Chapter 2.2 --- Larger scale EST analysis of black seabream pituitary cDNA libraries --- p.19 / Chapter 2.3 --- "Northern blot analysis of gene expression levels of growth hormone (GH), prolactin (PRL), somatolactin (SL) and P-actin in adult female black seabream pituitaries" --- p.23 / Chapter 2.4 --- Real time-PCR analysis of seasonal expression of growth hormone family genes in black seabream pituitary --- p.25 / Chapter 2.5 --- Dot blot (miniarray) analysis of seasonal expression of different genes expressed in black seabream pituitary --- p.28 / Chapter Chapter 3: --- Preliminary EST Analysis of Black Seabream Pituitary cDNA Libraries --- p.30 / Chapter 3.1 --- Results and discussion --- p.30 / Chapter 3.1.1 --- EST analysis of black seabream pituitary cDNA libraries --- p.30 / Chapter 3.1.2 --- Full-length sequencing of different hormone genes of black seabream --- p.47 / Chapter Chapter 4: --- Larger Scale EST Analysis of Black Seabream Pituitary cDNA Libraries --- p.64 / Chapter 4.1 --- Results and discussion --- p.64 / Chapter 4.1.1 --- EST analysis of black seabream pituitary cDNA libraries --- p.64 / Chapter 4.1.2 --- Genes first time found in fish --- p.91 / Chapter Chapter 5: --- Analysis of Black Seabream Pituitary Gene Expressions in Pituitary Collected from Different Months --- p.103 / Chapter 5.1 --- Introduction --- p.103 / Chapter 5.2 --- Northern blot analysis of seasonal variation of growth hormone family genes --- p.105 / Chapter 5.3 --- Seasonal analysis of growth hormone family genes by real time-PCR --- p.110 / Chapter 5.4 --- Sexual maturity of black seabream throughout the reproductive cycle --- p.118 / Chapter 5.5 --- Seasonal analysis of black seabream pituitary genes by dot blot study --- p.119 / Chapter 5.6 --- Conclusion --- p.153 / Chapter Chapter 6: --- General Discussion --- p.154 / References --- p.164
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_325243 |
| Date | January 2005 |
| Contributors | Kong, Pui Shan., Chinese University of Hong Kong Graduate School. Division of Biochemistry. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xi, 185 leaves : ill. (some col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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