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Expressed sequence tag (EST) analysis of black seabream (Acanthopagrus schlegeli) pituitary and seasonal analysis of gene expression in black seabream pituitary.

Kong Pui Shan. / Thesis submitted in: November 2004. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2005. / Includes bibliographical references (leaves 164-185). / Abstracts in English and Chinese. / Table of Contents --- p.i / List of Figures and Tables --- p.iii / List of Abbreviations --- p.viii / Chapter Chapter 1: --- Introduction --- p.1 / Chapter 1.1 --- Endocrine system and the pituitary gland in teleosts --- p.1 / Chapter 1.2 --- The biology of black seabream (Acanthopagrus schlegeli) --- p.4 / Chapter 1.3 --- Seasonal variation of gene expression in black seabream pituitary --- p.7 / Chapter 1.4 --- Expressed sequence tag (EST) analysis --- p.9 / Chapter 1.5 --- Basic Local Alignment Search Tool (BLAST) --- p.11 / Chapter 1.6 --- Aim of this study --- p.14 / Chapter Chapter 2: --- Materials and Methods --- p.15 / Chapter 2.1 --- Preliminary EST analysis of black seabream pituitary cDNA libraries --- p.15 / Chapter 2.2 --- Larger scale EST analysis of black seabream pituitary cDNA libraries --- p.19 / Chapter 2.3 --- "Northern blot analysis of gene expression levels of growth hormone (GH), prolactin (PRL), somatolactin (SL) and P-actin in adult female black seabream pituitaries" --- p.23 / Chapter 2.4 --- Real time-PCR analysis of seasonal expression of growth hormone family genes in black seabream pituitary --- p.25 / Chapter 2.5 --- Dot blot (miniarray) analysis of seasonal expression of different genes expressed in black seabream pituitary --- p.28 / Chapter Chapter 3: --- Preliminary EST Analysis of Black Seabream Pituitary cDNA Libraries --- p.30 / Chapter 3.1 --- Results and discussion --- p.30 / Chapter 3.1.1 --- EST analysis of black seabream pituitary cDNA libraries --- p.30 / Chapter 3.1.2 --- Full-length sequencing of different hormone genes of black seabream --- p.47 / Chapter Chapter 4: --- Larger Scale EST Analysis of Black Seabream Pituitary cDNA Libraries --- p.64 / Chapter 4.1 --- Results and discussion --- p.64 / Chapter 4.1.1 --- EST analysis of black seabream pituitary cDNA libraries --- p.64 / Chapter 4.1.2 --- Genes first time found in fish --- p.91 / Chapter Chapter 5: --- Analysis of Black Seabream Pituitary Gene Expressions in Pituitary Collected from Different Months --- p.103 / Chapter 5.1 --- Introduction --- p.103 / Chapter 5.2 --- Northern blot analysis of seasonal variation of growth hormone family genes --- p.105 / Chapter 5.3 --- Seasonal analysis of growth hormone family genes by real time-PCR --- p.110 / Chapter 5.4 --- Sexual maturity of black seabream throughout the reproductive cycle --- p.118 / Chapter 5.5 --- Seasonal analysis of black seabream pituitary genes by dot blot study --- p.119 / Chapter 5.6 --- Conclusion --- p.153 / Chapter Chapter 6: --- General Discussion --- p.154 / References --- p.164

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_325243
Date January 2005
ContributorsKong, Pui Shan., Chinese University of Hong Kong Graduate School. Division of Biochemistry.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xi, 185 leaves : ill. (some col.) ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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