Zheng Tingting. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2004. / Includes bibliographical references (leaves 98-124). / Abstracts in English and Chinese. / Abstract --- p.i / Acknowledgements --- p.iv / Table of Contents --- p.vi / List of Tables --- p.viii / List of Figures --- p.ix / Abbreviations --- p.xi / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Triamcinolone acetonide(TA) --- p.1 / Chapter 1.1.1 --- Application in ophthalmology --- p.1 / Chapter 1.1.2 --- Mechanism of anti-inflammatory effect --- p.2 / Chapter 1.1.3 --- Side effects of TA --- p.4 / Chapter 1.1.4 --- Toxicity of TA --- p.5 / Chapter 1.2 --- Retinal pigment epithelial (RPE) cell --- p.6 / Chapter 1.3 --- Mechanism of cell death --- p.8 / Chapter 1.3.1 --- Apoptosis --- p.9 / Chapter 1.3.2 --- caspase --- p.11 / Chapter 1.3.3 --- Mitogen-activated protein kinase (MAPK) --- p.13 / Chapter 1.3.4 --- Activator Protein-1 (AP-1) --- p.15 / Chapter 1.4 --- Potassium channel (K+）） --- p.15 / Chapter 1.4.1 --- Molecular structure of KAtp channel --- p.16 / Chapter 1.4.2 --- Regulation of Katp channel --- p.17 / Chapter 1.4.3 --- Pinacidil --- p.18 / Chapter 1.5 --- Calcium channel --- p.20 / Chapter 1.5.1 --- VDCCs and subtypes --- p.21 / Chapter 1.5.2 --- Calcium channel blocker --- p.22 / Chapter 1.5.3 --- Verapamil --- p.23 / Chapter 1.6 --- Study objectives --- p.25 / Chapter Chapter 2 --- Methodology --- p.37 / Chapter 2.1 --- Cell biology --- p.37 / Chapter 2.1.1 --- Materials --- p.37 / Chapter 2.1.1.1 --- Culture related material --- p.37 / Chapter 2.1.1.2 --- Drugs --- p.37 / Chapter 2.1.1.3 --- Cell line and instrument --- p.37 / Chapter 2.1.2 --- Preparations --- p.38 / Chapter 2.1.2.1 --- Working medium --- p.38 / Chapter 2.1.2.2 --- Drugs --- p.38 / Chapter 2.1.2.3 --- MTT solution --- p.39 / Chapter 2.1.3 --- Cell culture and treatment process --- p.40 / Chapter 2.1.3.1 --- Seed cell --- p.40 / Chapter 2.1.3.2 --- Treatment --- p.40 / Chapter 2.1.4 --- MTT-Cell Proliferation Assay --- p.41 / Chapter 2.2 --- Molecular biology --- p.42 / Chapter 2.2.1 --- Materials --- p.42 / Chapter 2.2.1.1 --- "Chemicals, reagents, and kits" --- p.42 / Chapter 2.2.1.2 --- Solutions and Buffers --- p.42 / Chapter 2.2.1.3 --- Primers and Enzymes --- p.43 / Chapter 2.2.1.4 --- Equipment --- p.43 / Chapter 2.2.1.5 --- Software --- p.43 / Chapter 2.2.2 --- Reverse transcription 226}0ؤ Polymerase Chain Reaction (RT-PCR) --- p.44 / Chapter 2.2.2.1 --- Cell collection and RNA Isolation --- p.44 / Chapter 2.2.2.2 --- Reverse Transcription (RT) --- p.45 / Chapter 2.2.2.3 --- PCR Reaction --- p.46 / Chapter 2.3 --- Immunocytochemistry --- p.48 / Chapter 2.3.1 --- Materials and instrumentation --- p.49 / Chapter 2.3.1.1 --- Antibodies and Equipment --- p.49 / Chapter 2.3.1.2 --- Chemicals and other useful items --- p.49 / Chapter 2.3.2 --- Preparations --- p.50 / Chapter 2.3.2.1 --- Preparation of coverslips --- p.50 / Chapter 2.3.2.2 --- Prepations of solutions --- p.50 / Chapter 2.3.3 --- Procedures --- p.51 / Chapter 2.4 --- Expression of results and statistics --- p.52 / Chapter Chapter 3 --- Results --- p.57 / Chapter 3.1 --- Effects of TA on RPE cell culture --- p.57 / Chapter 3.1.1 --- Cell morphology --- p.57 / Chapter 3.1.2 --- MTT assay --- p.57 / Chapter 3.1.3 --- Gene expressions --- p.57 / Chapter 3.2 --- Effects of PIN/VP on TA treated RPE cells --- p.58 / Chapter 3.2.1 --- MTT assay --- p.58 / Chapter 3.2.2 --- Gene expression --- p.59 / Chapter 3.2.2.1 --- Expression of housekeeping gene --- p.59 / Chapter 3.2.2.2 --- Expression of apoptosis-related gene --- p.59 / Chapter 3.2.2.3 --- Expression of early-response genes --- p.60 / Chapter 3.2.3 --- Immunofluorescence --- p.61 / Chapter Chapter 4 --- Discussion --- p.86 / Chapter Chapter 5 --- References --- p.98
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_324645 |
| Date | January 2004 |
| Contributors | Zheng, Tingting., Chinese University of Hong Kong Graduate School. Division of Ophthalmology and Visual Sciences. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xii, 124 leaves : ill. (some col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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