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Biopotencijal i hemijska karakterizacija ekstrakata i etarskih ulja vrsta roda Juniperus L. (Cupressaceae). / Biopotential and chemical characterization of extracts and essential oils of species from Juniperus L. genus (Cupressaceae).

<p>Karakterizacija metanolnih ekstrakata i etarskih ulja iglica i &scaron;i&scaron;arki osam vrsta samoniklih kleka (rod Juniperus L.) obuhvatala je fitohemijski skrining i ispitivanje antioksidantne, antiinflamatorne i antimikrobne aktivnosti. Primenom LC-MS/MS i GC-MS tehnike detektovan i odreĎen je sadržaj fenolnih i terpenoidnih sekundarnih biomolekula. Antioksidantna aktivnost ekstrakata i etarskih ulja (sposobnost neutralizacije slobodnih radikala, redukcioni potencijal i inhibicija lipidne peroksidacije) ispitana je primenom spektrofotometrijskih metoda. U cilju odreĎivanja antiinflamatornog potencijala, primenjena je ex vivo metoda za praćenje aktivnosti trombocitne ciklooksigenaze-1 i 12-lipoksigenaze. Antimikrobna aktivnost etarskih ulja odreĎena je na &scaron;est odabranih bakterijskih sojeva. Ispitani ekstrakti i etarska ulja pokazali su značajnu biolo&scaron;ku aktivnost.</p> / <p> Characterization of methanol extracts and essential oils of eight Juniperus L. species included phytochemical screening and evaluation of antioxidant, anti-inflammatory and antimicrobial activity. The presence and content of phenolics and terpenoids was confirmed by LC-MS/MS and GC-MS technique. Antioxidant activity of extracts and essential oils (radical scavenger capacity, reduction potential and inhibition of lipid peroxidation) was examined by spectrophotometric methods. With the intention to evaluate anti-inflammatory activity, an ex vivo method was applied to measure activity of platelet cyclooxygenase-1 and 12-lipoxygenase. Antimicrobial activity was evaluated according to six bacterial strains. Examined extracts and essential oils showed noticeable biological activity.</p>

Identiferoai:union.ndltd.org:uns.ac.rs/oai:CRISUNS:(BISIS)81464
Date23 November 2011
CreatorsLesjak Marija
ContributorsMimica-Dukić Neda, Kuhajda Ksenija, Beara Ivana, Božin Biljana, Anačkov Goran
PublisherUniverzitet u Novom Sadu, Prirodno-matematički fakultet u Novom Sadu, University of Novi Sad, Faculty of Sciences at Novi Sad
Source SetsUniversity of Novi Sad
LanguageSerbian
Detected LanguageEnglish
TypePhD thesis
Formatapplication/pdf

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