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Behavior of Acyl Carrier Proteins on Western Blots

Acyl carrier proteins (ACPs) from Escherichia coli and Euglena were analyzed on Western blots using rabbit antibodies raised against E. coli ACP. Euglena ACP, unlike that from E. coli, behaves upon electrophoresis under denaturing conditions as its size would predict. Oligomeric forms of both ACPs were evident on Western blots, but the bacterial ACP had more tendency to aggregate. That the oligomeric forms were not due to impurities was shown by their regeneration from low-Mr protein, reaction with antibodies isolated from Iow-Mr protein, and by molecular weight determination of the ACP by low-angle laser light scattering.

Identiferoai:union.ndltd.org:ETSU/oai:dc.etsu.edu:etsu-works-12726
Date02 April 1990
CreatorsWorsham, Lesa M., Tucker, Margie, Lou Ernst-Fonberg, M.
PublisherDigital Commons @ East Tennessee State University
Source SetsEast Tennessee State University
Detected LanguageEnglish
Typetext
SourceETSU Faculty Works

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