Psilocybe mutans sp. nov. had ceased fruiting consistently in artificial culture several years prior to the initiation of this study. In the couse of this study fruiting bodies were produced on a number of different substrates. All cultures were inoculated by mycelial transfer or by spraying with a suspension of pre-germinated spores. All cultrues were incubated for one to two weeks in a constant-temperature room at 70°F. on a shelf fourteen inches below a fluorescent lighting fixture. Lighting was continuous with an intensity at shelf level of approximately 3,000 foot-candles. Fruiting on any particular substrate was improved if the substrate contained a water extract of rabbit, horse, sheep, or cow dung. The basic media used most consistently in this study contained 0.5 grams MgSO4, 0.5 grams CaNO3, 0.25 grams KH2PO4, 5.0 grams maltose, 25.0 grams Difco agar and 1.0 liter distilled water. A variation of the basic media in which 0.25 liter of water extract of rabbit dung replaced 0.25 liter of water, was used in cultures to produce consistently good fruiting. Most cultures in this study utilized 100cc of agar media. The present strain of P. mutans ceased fruiting when several successive transfers of mycelium were cultured on the agar media given above, but containing no dung extract. Fruiting occurred again when
| Identifer | oai:union.ndltd.org:BGMYU2/oai:scholarsarchive.byu.edu:etd-9027 |
| Date | 01 December 1960 |
| Creators | Brown, Raymond C. |
| Publisher | BYU ScholarsArchive |
| Source Sets | Brigham Young University |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Theses and Dissertations |
| Rights | http://lib.byu.edu/about/copyright/ |
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