Wong Chi-Fai. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2002. / Includes bibliographical references (leaves 119-132). / Abstracts in English and Chinese. / Abstract --- p.i / Acknowledgements --- p.iv / List of Abbreviations --- p.v / List of Figures --- p.vii / List of Tables --- p.ix / Chapter 1 --- Introduction / Chapter 1.1 --- Plant secondary metabolites --- p.1 / Chapter 1.2 --- Plant alkaloids --- p.6 / Chapter 1.3 --- Narciclasine --- p.12 / Chapter 1.3.1 --- Isoquinoline alkaloids --- p.12 / Chapter 1.3.2 --- Amaryllidaceae alkaloids --- p.14 / Chapter 1.3.3 --- Narcissus --- p.16 / Chapter 1.3.4 --- Narciclasine --- p.17 / Chapter 1.3.4.1 --- Isolation --- p.17 / Chapter 1.3.4.2 --- Biological and pharmaceutical functions --- p.20 / Chapter 1.3.5 --- High performance liquid chromatography (HPLC) --- p.23 / Chapter 1.3.6 --- In vitro protein synthesis --- p.24 / Chapter 1.3.6.1 --- Rabbit reticulocyte lysate --- p.25 / Chapter 1.3.6.2 --- Wheat germ extract --- p.25 / Chapter 1.3.6.3 --- Non-radioactive colorimetric detection system --- p.26 / Chapter 1.4 --- Objective --- p.28 / Chapter 2 --- Materials and Methods / Chapter 2.1 --- Plant materials --- p.29 / Chapter 2.2 --- Extraction of narcicalsine --- p.29 / Chapter 2.3 --- Distribution of NCS in Narcissus tazetta --- p.30 / Chapter 2.4 --- Stability test --- p.31 / Chapter 2.4.1 --- HPLC analysis --- p.31 / Chapter 2.4.1.1 --- HPLC system --- p.31 / Chapter 2.4.1.2 --- Analytical condition --- p.31 / Chapter 2.4.2 --- Seed germination assay --- p.32 / Chapter 2.5 --- Mode of action of NCS --- p.33 / Chapter 2.5.1 --- In vitro translation --- p.33 / Chapter 2.5.1.1 --- In vitro translation --- p.33 / Chapter 2.5.1.2 --- SDS-PAGE analysis --- p.33 / Chapter 2.5.1.3 --- Western blot analysis --- p.34 / Chapter 2.5.1.4 --- Colorimetric detection --- p.34 / Chapter 2.5.2 --- Assay of induction of a-amylase synthesis in aleurone cells of barley grains by GA3 --- p.36 / Chapter 2.5.2.1 --- Chemicals and reagents --- p.36 / Chapter 2.5.2.2 --- Reducing sugar assay --- p.37 / Chapter 2.5.3 --- Root tip smear --- p.43 / Chapter 2.5.3.1 --- Chemicals and reagents --- p.43 / Chapter 2.5.3.2 --- Assay --- p.43 / Chapter 2.6 --- Allelopathic test --- p.45 / Chapter 2.6.1 --- Soil planting --- p.45 / Chapter 2.6.1.1 --- Foliage spray --- p.45 / Chapter 2.6.1.2 --- Planting with Narcissus bulb --- p.45 / Chapter 2.6.2 --- Hydroponics --- p.46 / Chapter 2.7 --- Effect of NCS on plant cells via tissue culture --- p.48 / Chapter 2.7.1 --- Establishment of tissue culture system --- p.48 / Chapter 2.7.1.1 --- Initiation and maintenance of carrot callus --- p.48 / Chapter 2.7.1.2 --- Initiation and maintenance of tobacco callus --- p.49 / Chapter 2.7.1.3 --- Initiation and maintenance of Narcissus callus --- p.50 / Chapter 2.7.1.4 --- Optimisation of callus growth --- p.50 / Chapter 2.7.2 --- Effects of NCS --- p.51 / Chapter 2.7.3 --- Effect of tobacco extract on NCS --- p.51 / Chapter 2.7.3.1 --- Extraction of tobacco extract --- p.51 / Chapter 2.7.3.2 --- Bioassay --- p.52 / Chapter 2.8 --- Assay of effect of NCS on microorganisms --- p.53 / Chapter 2.8.1 --- Antibacterial activity --- p.53 / Chapter 2.8.1.1 --- Total bacterial count --- p.53 / Chapter A. --- Chemicals and reagents --- p.53 / Chapter B. --- Serial dilution --- p.54 / Chapter C. --- Assay --- p.54 / Chapter 2.8.1.2 --- Turbidity test --- p.55 / Chapter A. --- Bacteria --- p.55 / Chapter B. --- Chemicals and reagents --- p.55 / Chapter C. --- Assay --- p.55 / Chapter 2.8.2 --- Anti-fungal and anti-yeast activity --- p.56 / Chapter 2.8.2.1 --- Disc diffusion method --- p.56 / Chapter A. --- Fungi --- p.56 / Chapter B. --- Chemicals and reagents --- p.56 / Chapter C. --- Assay --- p.56 / Chapter 2.8.2.2 --- Tube dilution method --- p.57 / Chapter A. --- Yeast --- p.57 / Chapter B. --- Chemicals and reagents --- p.57 / Chapter C. --- Assay --- p.57 / Chapter 2.9 --- Statistical analysis / Chapter 3 --- Results / Chapter 3.1 --- Distribution of NCS in Narcissus tazetta --- p.59 / Chapter 3.2 --- Stability of NCS --- p.62 / Chapter 3.2.1 --- HPLC analysis --- p.62 / Chapter 3.2.2 --- Bioassay --- p.62 / Chapter 3.3 --- Mode of action of NCS --- p.66 / Chapter 3.3.1 --- In vitro translation --- p.66 / Chapter 3.3.2 --- Effect ofNCS on the induction of a-amylase synthesis in aleurone cells of barley grains by GA3 --- p.69 / Chapter 3.3.3 --- Root tip smear --- p.74 / Chapter 3.4 --- Allelopathic test --- p.77 / Chapter 3.4.1 --- Soil planting --- p.77 / Chapter 3.4.1.1 --- Foliage applications --- p.77 / Chapter 3.4.1.2 --- Planting with Narcissus bulb --- p.77 / Chapter 3.4.2 --- Hydroponics --- p.78 / Chapter 3.5 --- Effect of NCS on plant cells via tissue culture --- p.91 / Chapter 3.5.1 --- Optimisation of Narcissus callus growth --- p.91 / Chapter 3.5.2 --- "Effects of NCS on Narcissus, carrot and tobacco calli" --- p.91 / Chapter 3.5.3 --- Effect of tobacco extract on NCS --- p.91 / Chapter 3.6 --- Effect of NCS on microorganisms --- p.95 / Chapter 3.6.1 --- Antibacterial activity --- p.95 / Chapter 3.6.1.1 --- Total bacterial count --- p.95 / Chapter 3.6.1.2 --- Turbidity test --- p.95 / Chapter 3.6.2 --- Anti-fungal and anti-yeast activities --- p.95 / Chapter 3.6.2.1 --- Disc diffusion method --- p.95 / Chapter 3.6.2.2 --- Tube dilution method --- p.96 / Chapter 4 --- Discussion / Chapter 4.1 --- General properties --- p.103 / Chapter 4.2 --- Mode of action --- p.105 / Chapter 4.3 --- Other biological properties --- p.108 / Chapter 4.3.1 --- Allelopathic property --- p.108 / Chapter 4.3.2 --- Effect on other plants via tissue culture --- p.110 / Chapter 4.3.3 --- Effect on microoraganisms --- p.112 / Chapter 4.4 --- Further studies --- p.114 / Chapter 5 --- Conclusion --- p.115 / Appendix --- p.116 / References --- p.119
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_324043 |
| Date | January 2002 |
| Contributors | Wong, Chi Fai, Chinese University of Hong Kong Graduate School. Division of Biology. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, ix, 132 leaves : ill. (some col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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