This thesis describes the isolation and characterization of four steroidal alkaloids from Buxus macowanii.
Phytochemical investigation of Buxus macowanii resulted in the isolation of two novel steroidal alkaloids, Nb-demethyl-6-deoxy-16-acetoxy O2-natafuranamin (112), and 6-deoxy-16-acetoxy O10-natafuranamin (113) alongside two known steroidal alkaloids, cycloprotobuxine-D (114), and cycloprotobuxine-F (115). Compounds 114 and 115 have been isolated for the first time from B. macowanii. Structure of compounds 112-115 was elucidated with aid of UV, IR, mass, and 1D and 2D NMR spectroscopy. These compounds showed different level of anti-AChE activity. Among all the isolates, compound 112 was found to be significantly active against AChE with an IC50 value of 4.7 µM. The bioactivity of this new compound nearly comparable to those of huperzine (IC50 = 1.7 µM) and O2-natafuranamine (IC50 = 3.0 µM).
| Identifer | oai:union.ndltd.org:MANITOBA/oai:mspace.lib.umanitoba.ca:1993/24080 |
| Date | 30 September 2014 |
| Creators | Almalki, Manal |
| Contributors | Athar, Ata (Chemistry), Frank, Schweizer (Chemistry) Rotimi Aluko (Human nutritional sciences) |
| Source Sets | University of Manitoba Canada |
| Detected Language | English |
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