Thesis (D.Sc.D.)--Boston University, Henry M. Goldman School of Graduate Dentistry, 1992 (Oral Biology). / Includes bibliographical references (leaves 70-84). / The ALG7 gene encodes the tunicamycin-sensitive, dolichol-P-dependent Nacetylglucosamine-
1-phosphate transferase, GPT, that catalyzes the synthesis of the first dolichollinked
sugar, Dol-PP-GlcNAc, in the N-glycosylation pathway. ALG7 has been evQlutionarily
conserved and is essential for growth in all eukaryotes. The ALG7 gene expression in yeast is known to be regulated in part by the 3' untranslated regions (UTR) of the ALG7 multiple
transcripts at the posttranscriptional level. To examine the regulatory features of the mammalian ALG7 gene, cloning and characterization of the hamster ALG7 mRNAs were undertaken.
Polymerase chain reaction (PCR) using a single ALG7 gene-specific primer was performed to
clone the cDNAs corresponding to the 3' and 5' ends of the ALG7 mRNAs from the Chinese
hamster ovary (CHO) cells. The initial Northern blot analysis using a hamster ALG7 genomic
DNA as a probe has shown that in the CHO cells the ALG7 gene is transcribed into three major messages, approximately 1.5, 1.9, and 2.2 kb in size. The 1.9 kb transcripts were cloned and sequenced. There is one consensus polyadenylation signal AAUAAA located 12 nucleotides (nt) upstream to the major poly(A) site. Three additional minor poly(A) sites are located at 18, 21 and 29 nt downstream from the AAUAAA sequence in this 1.9 kb class of mRNAs. [TRUNCATED]
| Identifer | oai:union.ndltd.org:bu.edu/oai:open.bu.edu:2144/31297 |
| Date | January 1992 |
| Creators | Huang, George T.-J. |
| Publisher | Boston University |
| Source Sets | Boston University |
| Language | en_US |
| Detected Language | English |
| Type | Thesis/Dissertation |
| Rights | This work is being made available in OpenBU by permission of its author, and is available for research purposes only. All rights are reserved to the author. |
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