The F1 generation, which was obtained by hybridization transgenic lines of malting barley SCLW-GP-PHYA with feed barley of varieties Azit, Heris and Tocada, was formed for the purpose of transmission of the phyA transgene, which controls the production of the enzyme phytase. 107 hybrids of F1 generation, where it feed variety Azit was used as male plants, has been verified hybrid origin using SSR EBmac0603. In 27 individuals have been confirmed hybrid origin and by 23 individuals has been showed by the method PCR of the phyA transgene. Based on the measurement of phytase activity in the caryopsis F1 generation, which was lower compared with the female transgenic lines, but higher than in male feed variety Azit. F2 generation was observed phenotypic cleavage in size plant, length spike and grain size. Positive plants of the phyA transgene have been used to stabilize transgene and obtained homozygous plants by method androgenesis. From 4834 anthers have been obtained 54 green plants and 148 albinotic plants The phyA transgene were by the method PCR in 33 green plants (61.6 %) and 2 albinotic plants (1.4 %). Flow cytometry has been used to determine to level of ploidy in 54 green plants and have been 42 doubled haploid (77.7 %), 6 haploid (11.1 %), 4 tetrahaploid (7.4 %) and 2 trihaploid (3.7 %). Reciprocal hybridization has been performed, when feed varieties were used as female plants and transgenic barley lines SCLW-GP-PHYA as a male plants. By 10 plants od F1 generation were by the method PCR the transgene phyA in 8 plants.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:180011 |
| Date | January 2014 |
| Creators | Hanáková, Marie |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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