<p>In this research we observed the
effects of basil on stress modulation and immune response in vertebrates <i>in vitro</i> and <i>in vivo</i>. </p>
<p>The <i>in vitro</i> study was done on mice spleen cell cultured with Holy
basil and Thai basil to observe their proliferation stimulation to spleen cells
and spleen T cell. The study was done in presence and absence of Concanavalin A
(Con A)- a mitogen known as a T cell proliferation stimulator. A single cell
suspension of mice spleen cells was incubated with four different dilutions of
Holy and Thai basil (1:1, 1:5, 1:25, and 1:125). No significant differences have
been found between control groups (cells without factor) and treatments
suggesting the basils did not show any stimulation to spleen cell or spleen T
cell proliferation. </p>
<p>The <i>in vivo</i> study was conducted with tilapia reared in aquaponics
system to observe the stress modulating property of Holy basil. The
physiological and immunological responses and growth performances of stressed
and basil fed tilapia have been observed. There were four groups of fish-
Control (unstressed) fish fed with commercial feed, Stressed fish fed with
commercial feed, Control (unstressed) fish fed with basil-supplemented
commercial feed, and Stressed fish fed with basil-supplemented commercial feed.
The experiment was six weeks long. The parameters which have been recorded
were- serum cortisol, packed cell volume, plasma protein, hepatosomatic index,
spleen-somatic index, lysozyme activity, macrophage phagocytic capacity,
length, weight, and condition factor. The results did not suggest any effects
of basil on stress response. But the harmful effect of stress on fish growth
and immune response was evident, as the stress groups showed significantly lower
length, weight and condition factor. </p>
<p>The aquaponics system used in this
study was also investigated for its production of the crops. Total fish
production after eight weeks of placing the fingerlings in the aquaponics
system were as follows- Control group: 6.00 g/L, Stressed group: 3.98 g/L. It
suggests that stress can result in far less profit in aquaponics production.</p>
<p>Total plant production after 5
months is 6,521.10 g/sq. m (leaf with stem of marketable size) for Holy basil
and 7219.73 g/sq. m for Thai Basil. This result suggests that basils are a
viable crop in aquaponics and Thai basil would be more productive as the plant
crop than Holy basil when grown on aquaponics.</p>
| Identifer | oai:union.ndltd.org:purdue.edu/oai:figshare.com:article/12241139 |
| Date | 05 May 2020 |
| Creators | Aparna R Biswas (8795681) |
| Source Sets | Purdue University |
| Detected Language | English |
| Type | Text, Thesis |
| Rights | CC BY 4.0 |
| Relation | https://figshare.com/articles/PHYSIOLOGICAL_AND_IMMUNOLOGICAL_EFFECTS_OF_BASIL_IN_VITRO_AND_IN_VIVO/12241139 |
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