The effect of interleukin-3 (IL-3), a candidate hemopoietic growth factor, on prenatal hemopoiesis is unclear. Microinjection of IL-3 directly into mouse fetuses (day 13) via the yolk sac, allowed us to evaluate the effects on morphogenetic events and more specifically on fetal liver populations using quantitative in vitro clonal assays for hemopoietic precursors. Control studies, required to distinguish stress effects of surgical laparotomy and microinjection, clearly revealed that the fetal liver is a sensitive organ responding with limited tissue disorganization, reduced cellularity and erythropoietic activity as identified 24 h after experimental intervention. Microinjection of 15 units of IL-3 promoted significant expansion of the depleted fetal liver hemopoietic cell populations and had stimulatory effects on connective tissue mast cells, absolute cell numbers including hemopoietic precursors (erythroid, granulocyte, macrophage, megakaryocyte) compared to controls. These studies suggest that fetal liver cells acquire a responsiveness to IL-3 early in development and that, IL-3 has a positive stimulatory effect on fetal liver cell populations, promoting the recovery of normal liver hemopoiesis.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.59630 |
| Date | January 1990 |
| Creators | Delorme, Danielle |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Anatomy.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001126959, proquestno: AAIMM66367, Theses scanned by UMI/ProQuest. |
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