This work focused on the natural development of neurotachykinin-1 (NK-1) receptors in the porcine brainstem during postnatal development (using 4 ages vs adult), and on alterations in these receptors after single and six-daily repeated clustered hypoxia (using young and older piglets). NK-1 receptor localization and densities were determined by quantitative autoradiography, using mono-iodinated Bolton-Hunter substance P ([125I]-BHSP). Slide-mounted brainstem sections, incubated in [125I]-BHSP and then exposed to film, have shown [125I]-BHSP binding throughout many brainstem nuclei and tracts, including the ambigual/periambigual (nAmb), dorsal motor vagal (dmnv), hypoglossal (nHyp), lateral reticular (nRL), gigantocellular (nGC), solitary tract (nTS), medial parabrachial (nPBM), and raphe obscurus (nROb) nuclei. NK-1 receptor densities decreased with age. As compared to normoxia, NK-1 receptor densities increased significantly after the 6-daily hypoxia protocol in dmnv, nHyp, nRL, nROb, and nTS of both young and older age groups. This increase may represent receptor upregulation as an adaptation to repeated hypoxia.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.29569 |
| Date | January 2002 |
| Creators | Rodier, Mitchell Ellis |
| Contributors | Moss, Immanula (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Physiology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001974330, proquestno: MQ85920, Theses scanned by UMI/ProQuest. |
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