This study tests the hypothesis that glucose tolerance in fish is related to nutrient preference and that this tolerance is correlated with hexokinase activity and tissue glucose transporters. Differences in glucose clearance abilities of three fish species, the carnivorous rainbow trout (Oncorhynchus mykiss) and American eel (Anguilla rostrata) and the omnivorous bullhead catfish (Ameiurus melas) were investigated in this study. Glucose tolerance was compared between the three species and between feeding and 6 month fasted eels. Glucose tolerance was assessed by administering an intravenous glucose tolerance test (IGTT), injecting 250 mg glucose/kg body weight and taking blood samples over 24 h. Feeding eel and catfish were found to be more glucose tolerant than fasting eel and rainbow trout. Glucose turnover was investigated in the rainbow trout during the glucose tolerance test and the rate of appearance of glucose did not decrease until 2 h after a glucose load which may contribute to the glucose intolerance seen. To test what factors might contribute to differences in glucose tolerance, two steps within the glucose uptake pathway were examined in white muscle. (Abstract shortened by UMI.)
| Identifer | oai:union.ndltd.org:uottawa.ca/oai:ruor.uottawa.ca:10393/8700 |
| Date | January 1999 |
| Creators | Legate, Nicola J. |
| Contributors | Moon, Thomas W., |
| Publisher | University of Ottawa (Canada) |
| Source Sets | Université d’Ottawa |
| Detected Language | English |
| Type | Thesis |
| Format | 100 p. |
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