Molecular genetic analyses focused on selected aspects of technological quality were carried out on 25 wheat genotypes with non-standard coloured caryopses. Occurrence of null alleles was observed for the GBSSI gene responsible for amylose synthesis, the so-called waxy gene. Null allele Wx-B1 was found in 20 % of the genotypes, other null alleles were not observed. Allelic state of Pina and Pinb genes affecting caryopsis hardness was analysed too. Allele identification of three genotypes was not successful. In the Pina case, only the wild-type allele Pina-D1a was present, in the Pinb wider variability was observed, 36 % of the genotypes carried wild-type Pinb-D1a allele, 40 % carried Pinb-D1b allele and 12 % Pinb-D1d allele. Instrumental hardness was measured and it correlated with the detected allelic state. Caryopses texture was determined based on the laser beam transmission through the caryopsis. Occurrence of mealy caryopses ranged from 0 % to 100 %. Caryopsis texture and hard-ness do not correlate neither on the instrumental nor on the genetic basis. Null allele Wx-B1 occurrence did not influence the caryopsis hardness nor their texture. Partial sequences of F3H a DFR structural genes of the anthocyanin biosynthetic pathway were obtained at five genotypes with blue, purple and white caryopses. Single nucleotide polymorphisms among individual gene copies were observed.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:426258 |
| Date | January 2019 |
| Creators | Štiasna, Klára |
| Source Sets | Czech ETDs |
| Language | Slovak |
| Detected Language | English |
| Type | info:eu-repo/semantics/doctoralThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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