by Chiu-lun Fok. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1991. / Includes bibliographical references. / List of Abbreviations --- p.i / Abstract --- p.iii / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- General Properties of Oxalysine --- p.1 / Chapter 1.1.1 --- Chemical Structure and Properties --- p.1 / Chapter 1.1.2 --- Biological Properties --- p.2 / Chapter 1.1.2.1 --- Antimicrobial Activity --- p.2 / Chapter 1.1.2.2 --- Antitumor Activity --- p.2 / Chapter 1.1.2.3 --- Immunomodulatory Activity --- p.5 / Chapter 1.1.2.4 --- Other Biological Properties --- p.5 / Chapter 1.1.3 --- Pharmacokinetics and Toxicity --- p.6 / Chapter 1.2 --- General Properties of Ribosome-Inactivating and Abortifacient Proteins --- p.8 / Chapter 1.2.1 --- Research History --- p.8 / Chapter 1.2.1.1 --- Ribosome-Inactivating Proteins --- p.8 / Chapter 1.2.1.2 --- Abortifacient Proteins --- p.9 / Chapter 1.2.2 --- Relationship between Ribosome- Inactivating Proteins and Abortifacient Proteins --- p.10 / Chapter 1.2.3 --- Distribution --- p.11 / Chapter 1.2.4 --- Physicochemical Characteristics --- p.12 / Chapter 1.2.5 --- Biological Properties --- p.13 / Chapter 1.2.5.1 --- Effect on Protein Synthesis --- p.13 / Chapter 1.2.5.2 --- Effect on the Immune System --- p.14 / Chapter 1.2.5.3 --- Cytotoxic and Antitumor Activities --- p.16 / Chapter 1.2.5.4 --- Termination of Pregnancy --- p.17 / Chapter 1.2.5.5 --- Antiviral Activity --- p.18 / Chapter 1.2.6 --- The Study on Luffaculin --- p.19 / Chapter 1.3 --- Aim of the Present Study --- p.20 / Chapter 1.3.1 --- Oxalysine --- p.20 / Chapter 1.3.2 --- Luffaculin --- p.20 / Chapter Chapter 2 --- Materials and Methods --- p.22 / Chapter 2.1 --- Materials --- p.22 / Chapter 2.2 --- Methods --- p.30 / Chapter 2.2.1 --- In Vivo Drug Treatment --- p.30 / Chapter 2.2.2 --- Isolation and Preparation of Cells --- p.30 / Chapter 2.2.2.1 --- Peritoneal Exudate Cells --- p.30 / Chapter 2.2.2.2 --- Spleen Cells --- p.30 / Chapter 2.2.2.3 --- Ficoll-Paque Separation of Lymphocytes --- p.31 / Chapter 2.2.2.4 --- Congo Red-Stained Yeast Cells --- p.31 / Chapter 2.2.3 --- Lymphocyte Transformation --- p.32 / Chapter 2.2.4 --- Haemolytic Plaque Assay --- p.33 / Chapter 2.2.5 --- Phagocytic Activity --- p.33 / Chapter 2.2.6 --- Macrophage-Mediated Cytostatic Activity --- p.34 / Chapter 2.2.7 --- Delayed Type Hypersensitivity (DTH) --- p.35 / Chapter 2.2.8 --- Production of and Assay for Interleukin-2(IL-2) --- p.36 / Chapter 2.2.9 --- Cytotoxicity of the Drugs on Various Cell Lines --- p.38 / Chapter 2.2.9.1 --- Trypan Blue Exclusion Method --- p.38 / Chapter 2.2.9.2 --- Neutral Red Uptake Method --- p.38 / Chapter 2.2.10 --- Cytostatic Effect of the Drugs on Various Cell Lines --- p.39 / Chapter 2.2.11 --- Evaluation of Antitumor Activity (In Vivo ) --- p.40 / Chapter 2.2.11.1 --- Tumor Size --- p.40 / Chapter 2.2.11.2 --- Survival Study --- p.40 / Chapter 2.2.12 --- TLC Analysis --- p.40 / Chapter 2.2.13 --- Preparation of Ribosome-Inactivating and Abortifacient Proteins --- p.41 / Chapter 2.2.13.1 --- Trichosanthin (TCS) --- p.41 / Chapter 2.2.13.2 --- Luffaculin (LFC) --- p.42 / Chapter 2.2.14 --- Protein Determination --- p.42 / Chapter 2.2.15 --- Statistical Analysis --- p.43 / Chapter Chapter 3 --- The Immunomodulatory and Antitumor Activities of Oxalysine (OXL) --- p.44 / Chapter 3.1 --- Introduction --- p.44 / Chapter 3.2 --- The Immunomodulatory Activity of Oxalysine --- p.46 / Results --- p.46 / Chapter 3.2.1 --- Effect of Oxalysine on the Proliferation of Mouse Splenocytes --- p.46 / Chapter 3.2.2 --- Effect of In Vitro Oxalysine Exposure on the Response of Murine Splenocytes to Mitogens --- p.46 / Chapter 3.2.3 --- Effect of In Vivo Oxalysine Treatment on the Response of Murine Splenocytes to Mitogens --- p.49 / Chapter 3.2.4 --- Effect of Oxalysine on Delayed Type Hypersensitivity (DTH) Response --- p.51 / Chapter 3.2.5 --- Effect of Oxalysine on the In Vitro Phagocytic Activity of Mouse Peritoneal Macrophages --- p.51 / Chapter 3.2.6 --- Effect of Oxalysine on Macrophage- Mediated Cytostatic Activity --- p.53 / Chapter 3.2.7 --- Effect of Oxalysine on the Humoral Response to SRBC --- p.55 / Discussion --- p.59 / Chapter 3.3 --- Mechanistic Studies on Inhibition of Mitogen´ؤ Induced Lymphocyte Transformation by Oxalysine --- p.62 / Results --- p.62 / Chapter 3.3.1 --- Lack of Direct Cytotoxic Effect of Oxalysine on Mouse Splenocytes In Vitro --- p.62 / Chapter 3.3.2 --- Effect of Oxalysine on the Kinetics of Con A-Induced Lymphoproliferative Response --- p.62 / Chapter 3.3.3 --- Time Course Studies on the Effect of Oxalysine on Mitogen-Induced Lymphocyte Transformation --- p.64 / Chapter 3.3.3.1 --- Preincubation of Oxalysine --- p.64 / Chapter 3.3.3.2 --- Delayed Addition of Oxalysine --- p.67 / Chapter 3.3.4 --- Effect of Exogenous IL-2 on the Oxalysine-Mediated Suppression of Lymphocyte Blastogenesis --- p.69 / Chapter 3.3.5 --- Effect of Oxalysine on the Activity of IL-2 Containing Medium to Maintain the Proliferation of the IL´ؤ2 Dependent CTLL-2 Cells --- p.73 / Chapter 3.3.6 --- Production of IL-2 from Splenocytes of Oxalysine´ؤTreated Mice --- p.75 / Chapter 3.3.7 --- The In Vitro Effect of Oxalysine on the Production of IL-2 from Con A-Activated Mouse Splenocytes --- p.75 / Discussion --- p.79 / Chapter 3.4 --- The Antitumor Activity of Oxalysine --- p.83 / Results --- p.83 / Chapter 3.4.1 --- Cytotoxicity of Oxalysine on Various Tumor Cell Lines --- p.83 / Chapter 3.4.2 --- Cytostatic Effect of Oxalysine on Various Tumor Cell Lines --- p.85 / Chapter 3.4.3 --- Effect of Oxalysine on the Survival of Tumor-Bearing Mice --- p.93 / Chapter 3.4.4 --- Effect of Oxalysine on the Growth of Transplantable Tumor Cells In Vivo --- p.95 / Discussion --- p.100 / Chapter 3.5 --- General Discussion --- p.102 / Chapter Chapter 4 --- The Immunomodulatory and Cytotoxic Properties of Luffaculin (LFC) --- p.104 / Chapter 4.1 --- Introduction --- p.104 / Chapter 4.2 --- The Immunomodulatory Activity of Luffaculin --- p.106 / Results --- p.106 / Chapter 4.2.1 --- Lack of Direct Cytotoxic Effect of LFC on Mouse Splenocytes In Vitro --- p.106 / Chapter 4.2.2 --- Effect of Luffaculin on the Proliferation of Mouse Splenocytes --- p.108 / Chapter 4.2.3 --- Inhibition of the Mitogen-Induced Lymphocyte Transformation by Luffaculin --- p.108 / Chapter 4.2.4 --- Effect of Luffaculin on Delayed Type Hypersensitivity (DTH) Response --- p.114 / Chapter 4.2.5 --- Primary Humoral Immune Response to SRBC in Luffaculin-Treated Mice --- p.114 / Chapter 4.2.6 --- Effect of Luffaculin on Phagocytosis of Macrophages In Vitro --- p.117 / Chapter 4.2.7 --- Effect of Luffaculin on Macrophage- Mediated Cytostatic Activity --- p.117 / Chapter 4.2.8 --- Production of Interleukin´ؤ2 from Splenocytes of Luffaculin-Treated Mice --- p.119 / Discussion --- p.122 / Chapter 4.3 --- The Cytotoxic and Cytostatic Effects of Luffaculin on Various Tumor Cell Lines --- p.125 / Results --- p.125 / Chapter 4.3.1 --- Cytotoxicity of Luffaculin on Various Tumor Cell Lines --- p.125 / Chapter 4.3.2 --- Cytostatic Effect of Luffaculin on Various Tumor Cell Lines --- p.127 / Discussion --- p.138 / Chapter 4.4 --- General Discussion --- p.140 / References --- p.143
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_318748 |
| Date | January 1991 |
| Contributors | Fok, Chiu-lun., Chinese University of Hong Kong Graduate School. Division of Biochemistry. |
| Publisher | Chinese University of Hong Kong |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, iv, 151 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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