Phytochemical composition, antioxidant activity, pigment stability, bioactive
properties, and in-vitro absorption of polyphenolics in acai fruit (Euterpe oleracea
Mart.) were investigated. Detailed characterization of phenolic compounds present in
acai fruit, acai fruit pulp, and a polyphenolic-enriched acai oil were conducted by HPLCESI-
MSn analyses and their stability and influence on antioxidant capacity determined.
Anthocyanins were predominant in acai fruits, which also contained several flavone and
flavonol glycosides, flavanol derivatives, and phenolic acids. In-vitro absorption and
antiproliferative effects of phytochemical extracts from acai pulp and acai oil were
determined as a function of chemical composition. Polyphenolic mixtures from both acai
pulp and acai oil extracts significantly inhibited HT-29 colon cancer cell proliferation,
also inducing the generation of reactive oxygen species. In-vitro intestinal absorption
using Caco-2 cell models demonstrated that phenolic acids and monomeric flavanol
derivatives are readily transported through cell monolayers in-vitro. The influence of polyphenolic cofactors on the stability of anthocyanins in acai
fruit under varying conditions of temperature and pH was evaluated. Significant time,
temperature, and pH-dependent anthocyanin losses were observed in all models, yet the
presence of phenolic acids, procyanidins, and flavone-C-glycosides had a positive
influence on anthocyanin stability. External addition of flavone-C-glycosides
significantly enhanced visual color, increased anthocyanin stability during exposures to
high pH or storage temperatures, and had comparable effects to those of a commercial
anthocyanin enhancer.
Anthocyanin polymerization reactions occurring during storage of acai fruit juice
models were investigated and potential mechanisms and reaction products identified.
Polymeric anthocyanin fractions contained several anthocyanin-flavanol adducts based
on cyanidin or pelargonidin aglycones and their presence was related to increased
anthocyanin sulfite bleaching resistance and to the appearance of large, unresolved peaks
in HPLC chromatograms. A reaction mechanism involving the nucleophilic addition of
anthocyanins in their hydrated form to flavanol carbocations resulting from cleavage of
interflavanic bonds was proposed for the formation of flavanol-anthocyanin adducts in
acai fruit juices. Antiproliferative activity and in-vitro absorption of monomeric and
polymeric anthocyanin fractions were also evaluated. Both fractions inhibited HT-29
colon cancer cell growth in a similar, concentration-dependent manner, yet in-vitro
absorption trials using Caco-2 intestinal cell monolayers indicated the presence of
anthocyanin polymers may influence anthocyanin absorption in acai fruit products.
| Identifer | oai:union.ndltd.org:tamu.edu/oai:repository.tamu.edu:1969.1/ETD-TAMU-2009-05-400 |
| Date | 2009 May 1900 |
| Creators | Pacheco Palencia, Lisbeth A. |
| Contributors | Talcott, Stephen T. |
| Source Sets | Texas A and M University |
| Language | English |
| Detected Language | English |
| Type | Book, Thesis, Electronic Dissertation, text |
| Format | application/pdf |
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