En el análisis farmacognóstico del extracto hidroalcohólico de la corteza de Triplaris americana L., se han evidenciado compuestos fenólicos: taninos (taninos condensados) y flavonoides. En el extracto hidroalcohólico y aislado amílico del hidrolizado del extracto hidroalcohólico desecado, se observaron picos de absorción, en longitudes de onda para los grupos hidroxilos y grupo aromático, mediante espectrofotometría Infrarrojo (IR-FT) y espectrofotometría UV-visible. Se desarrolló y validó la cuantificación de taninos del extracto hidroalcohólico, mediante espectrofotometría Ultravioleta-visible, donde el método resultó ser selectivo, lineal, preciso (repetible y reproducible) y exacto; y se obtuvieron como resultado para compuestos fenólicos totales: 13.5158 ± 0.1825 g de ácido tánico/ 100 g de corteza, para taninos: 11.8590 ± 0.5453 g de ácido tánico/ 100 g de corteza; y para taninos condensados: 1.62368 ± 0.0784 g de ácido tánico/ 100 g de corteza. Al evaluar la toxicidad a dosis única del extracto hidroalcohólico (2000 mg/ kg de masa corporal), no se produjo mortalidad ni se manifestaron síntomas indicativos de toxicidad en los animales. Se evaluó la actividad antioxidante del extracto hidroalcohólico, aplicando el método del DPPH obteniendo como concentración inhibitoria IC50 19.53 mg/mL, con resultado levemente superior comparado con el estándar Trolox; y 0.241 mg/mL equivalentes Trolox. En el ensayo de la actividad antimicrobiana del extracto hidroalcohólico en concentraciones de 3 mg/ disco, 4 mg/ disco y 6 mg/ disco, se obtuvo una respuesta antibacteriana selectiva sobre las bacterias Gram positivas evaluadas: Staphylococcus aureus y Bacillus subtilis; y ausencia de actividad sobre las bacterias Gram negativas y actividad antifúngica. / In the farmacognostic analysis of the hidroalcoholic extract of the bark of Triplaris americana L., has been evidenced phenolics compound: tannins and flavonoids. In the hydroalcoholic extract and amilic isolated of the hydrolyzed of the extract dried up hydroalcoholic, picks of absorption were observed, in wave longitudes for the groups oxidriles (-OH) and aromatic group, by means of Infrared infrared spectrophotometry and UV-visible spectrophotometry. It was developed and it validated the quantification of tannins of the hydroalcoholic extract, by means of Ultraviolet-visible spectrophotometry, where the method turned out to be selective, lineal, precise (repeat and reproduce) and exact; and they were obtained as a result for compound total phenolic: 13.5158 ± 0.1825 g of tannic acid/ 100 g of bark, for tannins: 11.8590 ± 0.5453 g of tannic acid/ 100 g of bark and for condensed tannins: 1.62368 ± 0.0784 g of tannic acid/ 100 g of bark. When evaluating the toxicity to unique dose of the hydroalcoholic extract (2000 mg/kg of corporal mass), mortality didn't take place neither they showed indicative symptoms of toxicity in the animals. The antioxidant activity of the extract hydroalcoholic was evaluated, applying the method of the DPPH obtaining as inhibitory concentration IC50 19.53 g/mL, with slightly superior result compared with the standard Trolox; and 0.241 mg/mL equivalent Trolox. In the rehearsal of the antimicrobial activity from hydroalcoholic extract in concentrations of 3 mg/ disc, 4 mg/ disc and 6 mg/ disc, a selective antibacterial answer was obtained on the bacterial evaluated Gram positive: Staphylococcus aureus and Bacillus subtilis; and activity absence on the bacterial Gram negative and activity anti-yeast.
| Identifer | oai:union.ndltd.org:Cybertesis/sdx:www.cybertesis.edu.pe:80:sisbib/documents/sisbib.2009.inocente_cm-principal |
| Date | January 2009 |
| Creators | Inocente Camones, Miguel Angel |
| Contributors | urado Teixeira, Bertha J |
| Publisher | Universidad Nacional Mayor de San Marcos. Programa Cybertesis PERÚ |
| Source Sets | Universidad Nacional Mayor de San Marcos - SISBIB PERU |
| Language | Spanish |
| Detected Language | English |
| Format | text/xml |
| Rights | Inocente Camones, Miguel Angel |
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