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Biohemijska i fiziološka karakterizacija klonovatopole (Populus spp.) u procesu fitoekstrakcije bakra, nikla i kadmijuma / Biohemical and physiological characterization of three poplar clones (Populus spp.) during the copper, nickel and cadmium phytoextraction process

<p>Predmet ovog istaživanja&nbsp; bio je ispitivanje&nbsp;uticaja jona tri te&scaron;ka metala (Ni<sup>2+</sup>, Cu<sup>2+&nbsp;</sup>i Cd<sup>2+</sup>)&nbsp;u dve toksične koncentracije u zemlji&scaron;tu na&nbsp;fiziolo&scaron;ke i biohemijske karakteristike&nbsp;<br />odabranih klonova topola, M1, B229 i Pe 19/66.&nbsp;Ispitan je i potencijal odabranih klonova topola&nbsp;da vr&scaron;e fitoekstrakciju-akumulaciju te&scaron;kih&nbsp;metala iz zemlji&scaron;ta u svoje nadzemne delove &scaron;to&nbsp;dovodi&nbsp; do dugoročnog uklanjanja ovih&nbsp;perzistentnih polutanata iz životne sredine.&nbsp;Takođe, ispitan je uticaj te&scaron;kih metala na&nbsp;antioksidantni potencijal, sposobnost&nbsp;<br />neutalizacije slobodnih radikala, aktivnosti&nbsp;antioksidantih enzima kao i&nbsp; na sadržaj&nbsp;slobodnih i konjugovanih poliamina (Put, Spm,&nbsp;Spd),&nbsp; određenih HPLC analizom,&nbsp; i sadržaj&nbsp;biljnih hormona poput indol-3-sirćetne kiseline&nbsp;i&nbsp; abscisinske &nbsp;kiseline,&nbsp; određenih GC/MS&nbsp;analizom, u listovima i korenovima klonova topola.</p><p>&nbsp;</p> / <p>The aim of this study was to estimate and compare phytoextraction capacities of three&nbsp;poplar clones (M1, B229 and Pe 19/66) in soil. Furthermore, the goal was to assess &nbsp;different biological responses among the poplar clones during exposure to different concentration of three heavy metal ions (Ni<sup>2+</sup>, Cu<sup>2+</sup> i Cd<sup>2+</sup>). In order to track changes in&nbsp; poplars&rsquo;mineral, physiological, biochemical and antioxidant status during the&nbsp; abiotic stress, quantification of physiological properties, free and conjugated polyamines, total phenolics&nbsp; as well as quantification of phytohormones (indol-3-acetic and abscisic acid) was done. Furthermore, assessment of antioxidant potential by tracking radical scavenger capacities (RSC) against DPPH, ABTS, OH and NO radicals and by measuring enzymes activities (SOD, GSH-Px, GPx, GR) in vitro was performed in root and leaves of poplar clones.</p>

Identiferoai:union.ndltd.org:uns.ac.rs/oai:CRISUNS:(BISIS)89925
Date12 December 2014
CreatorsKebert Marko
ContributorsMimica-Dukić Neda, Štajner Dubravka, Malenčić Djordje, Orlović Saša, Orčić Dejan
PublisherUniverzitet u Novom Sadu, Prirodno-matematički fakultet u Novom Sadu, University of Novi Sad, Faculty of Sciences at Novi Sad
Source SetsUniversity of Novi Sad
LanguageSerbian
Detected LanguageEnglish
TypePhD thesis

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