<p>As the human population increases and the demand for
aquaculture increases, aquaculturists are coming up with new ways to mitigate
stress in fish to increase their production. Cannabidiol (CBD) is an up and
coming nutraceutical that may have potential to reduce stress in not only
humans but also other vertebrates such as fish. In this project the effect of
CBD on the stress physiology and immunology of Nile tilapia was evaluated both <i>in-vitro</i>
and <i>in-vivo</i>. In the <i>in-vitro</i> study, spleen cell proliferation was
conducted to observe the effect of CBD on fish T and B cells and were compared
to mouse T and B cell proliferation. In the <i>in-vivo</i> study, the fish were
reared in a recirculating aquaculture system. The effect of CBD on the stress
physiology of the fish in short term and long term were evaluated. Based on the
short-term acute study, a longer chronic study was designed where tilapia were
fed with and without CBD (0.001% of feed weight) and with and without
hydrocortisone stress hormone (0.01% of body weight) every day for four weeks.
This experiment compared the various growth and feed utilization parameters as
well as physiological and immunological parameters such as, plasma cortisol,
blood glucose and protein levels, liver and spleen somatic indices (HSI and SSI,
respectively), and lysozyme activity of the fish. From our current research,
CBD shows potential in stress modulation and in immune modulation. It may have
different effects based on the species, whether they need to enhance their
immune response or reduce inflammation to be healthy. It also seems to have had
different effect on different parts of the immune system. Hematological
parameters were not significantly affected by acute stress. CBD did not make
any substantial difference in growth. However, in the presence of stress, CBD
was able to lower lysozyme activity down to the normal control levels. By
administering the proper dosage of CBD on a case by case basis, health benefits
can be achieved. Further investigation into the matter may not just be useful
in stress mediation in aquatic organisms but may also have implications in
human medicine as well.</p>
| Identifer | oai:union.ndltd.org:purdue.edu/oai:figshare.com:article/14484819 |
| Date | 26 April 2021 |
| Creators | Asif Mortuza (10700823) |
| Source Sets | Purdue University |
| Detected Language | English |
| Type | Text, Thesis |
| Rights | CC BY 4.0 |
| Relation | https://figshare.com/articles/thesis/THE_EFFECT_OF_CANNABIDIOL_CBD_ON_THE_PHYSIOLOGY_AND_IMMUNOLOGY_OF_NILE_TILAPIA_OREOCHROMIS_NILOTICUS_IN-VITRO_AND_IN-VIVO/14484819 |
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