The purification of pepsinogen from the stomach of red perch using aqueous two phase systems (ATPS) formed by polyethylene glycol (PEG) and salt at 4°C was optimized. Salt type, salt concentration, PEG molecular weight and PEG concentration had significant effects on total volume (TV), volume ratio (VR), enzyme activity (AE), protein content (CP), specific activity (SA), purification fold (PF) and recovery yield (RY). (NH4)2SO4 at 15% w/w concentration was selected as the optimum salt type and concentration. PEG 1500 at 18% w/w concentration was selected as the optimum PEG molecular weight and concentration. 15% (NH4)2SO4-18% PEG 1500, the optimal ATPS, was compared with ammonium sulfate fractionation (ASF). ATPS gave better partition of pepsinogen (SA of 5.40 U/mg, PF of 5.20 and RY of 86.6%) than ASF (SA of 2.55 U/mg, PF of 2.46, RY of 70.4%). / This is the electronic copy of partition of pepsinogen in aqueous two phase system method.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:NSHD.ca#10222/14361 |
| Date | 29 November 2011 |
| Creators | Zhao, Lisha |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
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