The Nanoarchaeota were proposed as the fourth archaeal sub-division in 2002, and the only fully characterized nanoarchaeon was found to exist in a symbiotic association with the crenarchaeote, Ignicoccus sp. This nanoarchaeote, named Nanoarchaeum equitans could not be detected with &ldquo / universal&rdquo / archaeal 16S PCR primers and could only be amplified using specifically designed primers. In order to identify and access a wide diversity of archaeal phylotypes a new set of &ldquo / universal&rdquo / archaeal primers A571F (5&rsquo / -GCY TAA AGS RIC CGT AGC-3&rsquo / ) and UA1204R (5&rsquo / -TTM GGG GCA TRC IKA CCT-3&rsquo / ) was designed, that could amplify the 16S rRNA genes of all four archaeal sub-divisions. Using these primers community DNA was amplified from Chinese and New Zealand hydrothermal systems.
| Identifer | oai:union.ndltd.org:UNWC/oai:UWC_ETD:http%3A%2F%2Fetd.uwc.ac.za%2Findex.php%3Fmodule%3Detd%26action%3Dviewtitle%26id%3Dinit_9219_1174649966 |
| Date | January 2005 |
| Creators | Galada, Ncebakazi |
| Source Sets | Univ. of Western Cape |
| Language | English |
| Detected Language | English |
| Type | Theses and Dissertations |
| Format | |
| Coverage | ZA |
| Rights | Copyright: University of the Western Cape |
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