Arginase has been found to occur in the tentacles, gut, and body wall of Pista pacifica Berkeley_ Partially purified arginase from the intestine has a molecular weight of 200,000, a Km of about 155 mM, an arginase/canavanase ratio of 22, a pH optimum of 10.5, and a temperature optimum of 60°C. In addition, P. pacifica arginase is competitively inhibited by ornithine but is not inhibited by high arginine concentrations, nor by sulfhydryl reagents. The enzyme is not stimulated by exogenous manganese and breaks down into an active subunit under harsh treatment. The subunit has a KID of about 118 mM and is also unaffected by exogenous manganese.
Polychaete arginase shows most of the properties characteristic of arginases from other animal and plant species. However, none of the characteristics observed to date can be correlated with a particular mode of nitrotelism.
| Identifer | oai:union.ndltd.org:pdx.edu/oai:pdxscholar.library.pdx.edu:open_access_etds-2659 |
| Date | 01 January 1973 |
| Creators | O'Malley, Karen Laurel |
| Publisher | PDXScholar |
| Source Sets | Portland State University |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Dissertations and Theses |
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