The Pseudomonas aeruginosa ATCase was cloned and sequenced to determine the correct size, subunit composition and architecture of this pivotal enzyme in pyrimidine biosynthesis. During the course of this work, it was determined that the ATCase of Pseudomonas was not 360,000 Da but rather present in a complex of 484,000 Da consisting of two different polypeptides (36,000 Da and 44,000 Da) with an architecture similar to that of E. coli ATCase, 2(C3):3(r2). However, there was no regulatory polypeptide found in the Pseudomonas ATCase.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc278859 |
| Date | 05 1900 |
| Creators | Vickrey, John F. (John Fredrick), 1959- |
| Contributors | O'Donovan, Gerard A., Shanley, Mark Stephen, Kunz, Daniel A., Schwartz, Martin, Benjamin, Robert C., Stevens, L. Robert |
| Publisher | University of North Texas |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | vii, 206 leaves: ill., Text |
| Rights | Public, Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved., Vickrey, John F. (John Fredrick), 1959- |
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