The importance of chemical cues in triggering the settlement of marine invertebrate larvae has long been recognised but very few such cues have been definitively identified. Larvae of the Australian sea urchin Holopneustes purpurascens, which lives enmeshed in the fronds of macroalgae, are induced to settle by a water-soluble cue produced by the host alga Delisea pulchra. This cue was previously identified as a floridoside-isethionic acid complex. I present evidence in this thesis which supports histamine as the true settlement cue for larval H. purpurascens. The settlement cue was isolated from the polar extract of D. pulchra by bioassay-guided cation-exchange chromatography and identified as histamine using nuclear magnetic resonance spectroscopy. Algal derived and synthetic histamine at ~5 ??M induced rapid settlement in 80???100 % of larval H. purpurascens. In the first study of its kind for any marine invertebrate, variation in the distribution of new recruits was compared with quantitative variation in the distribution of histamine in the habitat. More than 90 % of new recruits were found on either the foliose red alga D. pulchra or on coralline turf algae. These algae induced >90 % settlement of larvae in laboratory assays after 24 h. D. pulchra contained far higher levels of histamine than all other algae, however, the coralline algae lacked measurable histamine. Seawater collected in situ adjacent to D. pulchra induced up to 16 % settlement of older larvae and contained the highest concentration of histamine (~5 nM). With the exception of coralline algae, variation in settlement and recruitment was consistent with the variation among species histamine contents. Initial results supported a biofilm derived settlement cue from coralline algae. I also showed that older H. purpurascens larvae settle in response to lower concentrations of histamine than younger larvae and required less exposure to histamine (10 ??M) in order to initiate irreversible metamorphosis. Histamine induced settlement of two other echinoids with non-feeding larvae. Histamine did not induce settlement of feeding larvae of two echinoids or settlement of non-feeding larvae of asteroids. Histamine may be a general settlement cue for echinoids with direct development.
| Identifer | oai:union.ndltd.org:ADTP/257240 |
| Date | January 2006 |
| Creators | Swanson, Rebecca, School of Biological, Earth & Environmental Sciences, UNSW |
| Publisher | Awarded by:University of New South Wales. School of Biological, Earth and Environmental Sciences |
| Source Sets | Australiasian Digital Theses Program |
| Language | English |
| Detected Language | English |
| Rights | Copyright Rebecca Swanson, http://unsworks.unsw.edu.au/copyright |
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