Macrotroponin, formed by the complexation of endogenous cardiac troponin autoantibodies with circulating cardiac troponin, poses a challenge in high-sensitive assays generating high values without cardiac damage. Treatment of blood samples with polyethylene glycol (PEG) precipitates biomacromolecules, including macrotroponin, thereby freeing up smaller molecules, such as monomeric troponin in solution. While previous studies have predominantly examined the impact on recovery of PEG-precipitation on troponin I assays, this pilot study sought to explore its effects on routine samples using a troponin T assay and how the PEG-precipitation method affects the recovery of troponins and other proteins of varying sizes, including ferritin, albumin, TSH, cystatin C, prolactin, IgA, IgM, and IgG. PEG-precipitation was employed as the separation method and conducted on 20 different troponin T samples, with the yield of troponin and other proteins examined, and also on a series of dilutions of one and the same sample with initially high troponin concentration. To estimate the robustness of the PEG precipitation method, all samples were measured as duplicates. For both the 20 individual samples and the diluted sample, the recovery of troponin decreased significantly with increasing troponin concentrations. This has not been previously documented in the literature. The finding implies that when employing PEG-precipitation to identify or rule out antibody-induced interference in troponin assays, it is imperative to account for the total concentration to evaluate the exchange rate. Furthermore, the PEG-precipitation method seems quite robust according to the low coefficient of variation for duplicate measurement.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:uu-530826 |
| Date | January 2024 |
| Creators | Rouf, Nma |
| Publisher | Uppsala universitet, Universitetsbiblioteket, Uppsala universitet, Institutionen för medicinsk cellbiologi |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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