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Effect of Inorganic Phosphate on the Morphology and Poly-β-Hydroxybutyrate Content of Azotobacter Vinelandii Atcc 12837

This study was designed to determine the effect of inorganic phosphate in the growth media of A. vinelandii on the synthesis and accumulation of poly-β-hydroxybutyrate (PHB) and cellular morphology. Burk's media with no added phosphate and ten times normal phosphate resulted in altered cells, lower levels of protein, and decreased cell populations. PHB accumulation was much higher in the cultures with no added phosphate; however, the polymer granules differed in appearance from those of normal cells and had a decreased rate of utilization. The amount of PHB deposited in cells grown in media containing ten times the normal phosphate level was lower than that found in regular media.

Identiferoai:union.ndltd.org:unt.edu/info:ark/67531/metadc798266
Date05 1900
CreatorsCollins, Patricia Ann
PublisherNorth Texas State University
Source SetsUniversity of North Texas
LanguageEnglish
Detected LanguageEnglish
TypeThesis or Dissertation
Formatvi, 49 leaves : ill., Text
RightsPublic, Collins, Patricia Ann, Copyright, Copyright is held by the author, unless otherwise noted. All rights

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