Respiratory system of the cystic fibrosis patients is affected by the defect in gene coding for protein transporter for chloride ions - CFTR ("Cystic fibrosis transmembrane conductance regulator"). The main complication of this disease is airways chronic inflammation, in particular caused by bacterium Pseudomonas aeruginosa. Due to asialylation of the lung surfaces the bacterial adhesion is facilitated, for example via lectin PAIIL. The ability of the chicken yolk antibodies to protect lung epithelial cells against Pseudomonas aeruginosa adhesion has been already proven. Therefore this thesis has mainly focused on the influence of the yolk antibodies specific against PAIIL on the development of infection in lungs of experimental animals. The objective was the optimization of the experimental model on which it would be possible to observe the infection development caused by luminescent bacteria strain in vivo using the optical tomography. At first the experiments have been performed on Wistar rats. Since the bacteria colonies in the rat lungs were not detectable in vivo on the available equipment, the rat experimental model showed up as not suitable. Further on only the mouse models were used. Experiments for the inhalation of the antibodies and intratracheal instillation of the bacteria suspension...
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:342930 |
| Date | January 2015 |
| Creators | Hadrabová, Jana |
| Contributors | Hodek, Petr, Nosková, Libuše |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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