Supplementation of a minimal medium with KHCO$ sb3$ and some amino acids reduced the duration of the lag period for the growth of the marine bacterium Deleya aesta 134 at sub-optimal Na$ sp+$ concentrations and decreased the minimal Na$ sp+$ concentration allowing growth, to 8mM. / Na$ sp+$ was required for the transport of most metabolites into D. aesta 134, while some compounds were taken up by both a Na$ sp+$-dependent and a Na$ sp+$-independent system. Phosphate transport was not affected by Na$ sp+.$ Na$ sp+$ was also required for the oxidation of exogenous succinate. Evidence for the presence in D. aesta 134 of a Na$ sp+$-activated NADH: quinone acceptor oxidoreductase was obtained. / A mutant of D. aesta 134 exhibiting a shorter lag period than its parent strain at 10mM Na$ sp+$ was also examined. Enhanced Na$ sp+$/H$ sp+$ antiport activity was obtained in the mutant strain, but the actual mechanism involved in the adaptation to low Na$ sp+$ concentrations is still obscure. / Succinate transport by D. aesta 134 was mediated by a C$ sb4$-dicarboxylate transport system, and exhibited biphasic kinetics, indicating the presence of a high- and a low-affinity transport system. / A partial genomic library of D. aesta was prepared, and DNA from D. aesta complemented mutations from Escherichia coli auxotrophs. Successful introduction of the plasmid pRK404 into D. aesta 134 by electroporation was also obtained.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.39451 |
| Date | January 1992 |
| Creators | Berthelet, Marc |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Microbiology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001304980, proquestno: NN80453, Theses scanned by UMI/ProQuest. |
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