The purpose of the present investigation was to demonstrate transformation in the genus Rhizobium, using strains of R. meliloti and R. trifolii as the test organisms. The initial work was directed towards an understanding of the metabolism of these organisms and the selection of stable mutants suitable for use in such a study. Five chloramphenicol-resistant strains of R. trifolii were obtained by a "Crystal Violet-tolerance" technique developed during the course of the present research. Suitably resistant strains of R. meliloti could not be isolated. Streptomycin resistance was subsequently selected for among the chloramphenicol-resistant strains.
Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.112803 |
Date | January 1960 |
Creators | Ellis, Norma. J. |
Contributors | Kalz, G. (Supervisor) |
Publisher | McGill University |
Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
Language | English |
Detected Language | English |
Type | Electronic Thesis or Dissertation |
Format | application/pdf |
Coverage | Doctor of Philosophy. (Department of Biology.) |
Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
Relation | alephsysno: NNNNNNNNN, Theses scanned by McGill Library. |
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