The synthesis of acetylcholine (ACh) was first demonstrated by Quastel, Tennenbaum and Wheatley in 1936 and by Mann, Tennenbaum and Quastel in 1938 and 1939 in rat brain and rat diaphragm slices and independently by Stedman and Stedman in 1937 and 1939 in rat brain homogenates. Feldberg in 1942, as did other early workers, confirmed the synthesis of acetylcholine in tissue slices and in the cellular debris of homogenates; the enzyme involved apparently remained fixed to the tissue or debris and was not obtained in solution. The enzyme, “choline acetylase”, was first obtained as a “cell-free” extract from rat brain and electric organ (Electriphorous electricus) homogenates by Nachmansohn and Machado (1943). “Choline acetylase” was extracted from peripheral nerve (Nachmansohn and John, 1945) and more recently in a highly active form from squid head ganglia homogenates and dried preparations (Nachmansohn and Weiss, 1948). Nachmansohn and his co-workers (1945b, 1946) concluded that the enzyme is present in all nerve tissue free of cell bodies and nerve endings, i.e., is present in motor and sensory nerves as well as in the optic nerve. Feldberg and Mann (1946) consider it to be absent from non-cholinergie nerve (3) fibres, i.e. from the posterior roots and the optic nerve. “Choline acetylase” has been demonstrated in heart auricular tissue (Bülbring and Burn, 1949) and in striated muscle (Nachmansohn, Barman and Weiss, 1947). [...]
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.123994 |
| Date | January 1952 |
| Creators | Girvin, Grace T. |
| Contributors | Stevenson, J. (Supervisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy. (Department of Bacteriology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: NNNNNNNNN, Theses scanned by McGill Library. |
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