The subfornical organ (SFO) is an area in the brain characterized by lack of a blood-brain-barrier that contributes to interaction between the circulation and the central nervous system, and plays key roles in regulation of energy balance. The SFO has two subregions- the dorsolateral peripheral SFO (pSFO) and the ventromedial core of the SFO (cSFO). This study demonstrated the expression of voltage-gated Na+ channel 1.3 (Nav1.3) in the SFO neurons, and a higher Nav1.3 expression in the pSFO. Based on the Nav1.3 expression pattern, intrinsic electrophysiological properties were compared between cSFO and non-cSFO neurons (putative pSFO neurons) identified by a SmartFlare mRNA probe. The patch clamp results revealed a bursting firing pattern in cSFO neurons and a higher spontaneous neuronal activity in non-cSFO neurons. The higher neuronal activity might be related to a more depolarized resting membrane potential and a higher trend of Na+ current density.
| Identifer | oai:union.ndltd.org:MANITOBA/oai:mspace.lib.umanitoba.ca:1993/24091 |
| Date | 09 October 2014 |
| Creators | Huang, Shuo |
| Contributors | Fry, Mark (Biological Sciences), Treberg, Jason (Biological Sciences) Aukema, Harold (Human Nutritional Sciences) |
| Source Sets | University of Manitoba Canada |
| Detected Language | English |
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