With a growing global population, food security and waste management strategies are needed (FAO, 2002b); insects have been promoted for these goals. Black soldier fly larvae (BSFL) are endorsed for their ability in decomposition and use as feed (Bava et al., 2019; Swett Walker, 2018). Studies show the life cycle of BSFL is impacted by bacterial supplementation (Franks et al., 2021; Kooienga et al., 2020). We seek to determine the effects of supplementing BSFL diet with Pseudomonas putida 3P. We conducted two forms of a 10-day bench-top study observing larval mass, frass conversion, C:N content, and ammonia production with supplementation of Pseudomonas putida 3P in single and double-doses. Supplementation with P. putida results in roughly 1.5X larger larval wet and dry mass, variable concentrations of ammonia, and approximately 1.15X smaller C:N ratios in frass. This suggests interruption of larval rearing may affect volatile ammonia concentrations and inhibit P. putida behavior.
| Identifer | oai:union.ndltd.org:MSSTATE/oai:scholarsjunction.msstate.edu:td-6519 |
| Date | 13 May 2022 |
| Creators | Waters, Rebecca |
| Publisher | Scholars Junction |
| Source Sets | Mississippi State University |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Theses and Dissertations |
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