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Identification and confirmation of molecular markers and orange flesh color associated with major QTL for high beta-carotene content in muskmelon

Beta-carotene presence or absence in muskmelon is controlled by two genes, green
flesh gf and white flesh wf. In its dominant form the wf gene is responsible for orange
flesh color; however, the epistatic interactions of gf and wf can create three flesh colors:
orange, white and green. Two F2 populations, consisting of 77 greenhouse grown and 117
field grown plants, from the cross of ‘Sunrise’ (white fleshed) by ‘TAM Uvalde’ (orange
fleshed), were used to examine the relationships of beta-carotene content, flesh color, and
flesh color intensity. Bulk segregent analysis was used with RAPD markers to identify
molecular markers associated with high beta-carotene content. Flesh color and flesh color
intensity both had significant relationships with beta-carotene content. A significant
correlation between total soluble solids and beta-carotene content was also found.
Molecular markers were identified in both F2 populations and all significant, associated
markers from ‘TAM Uvalde’ were linked with WF. A single QTL was also found to be
linked with the WF locus. The identified QTL can be used to screen potential breeding
lines for high beta-carotene. It was also confirmed that the visual ratings of flesh color
intensity can be reliably used to select high beta-carotene content melons.

Identiferoai:union.ndltd.org:tamu.edu/oai:repository.tamu.edu:1969.1/ETD-TAMU-1136
Date15 May 2009
CreatorsNapier, Alexandra Bamberger
ContributorsCrosby, Kevin M, Pike, Leonard M
Source SetsTexas A and M University
Languageen_US
Detected LanguageEnglish
TypeBook, Thesis, Electronic Thesis, text
Formatelectronic, application/pdf, born digital

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