Mediated microbial sensors utilizing two different yeast isolates (SPT1 and SPT2) were developed for the estimation of biochemical oxygen demand (BOD). Measurements of glucose/glutamic acid (GGA) standard solution with potassium ferricyanide mediation resulted in linear ranges extending from the detection limits (i.e. 2 and 5 ppm BOD) to 100 and 200 ppm BOD for the SPT1- and SPT2-based sensors, respectively. The standard error of the mean (SEW for 10 ppm. BOD measurements was 10.1% (SPT1) and 3.9% (SPT2). Response reproducibility had 10.6% error between three identically prepared SPT1 sensors. Response times for concentrations of 20 ppm BOD were within 10 minutes. For pulp mill effluent, the detection limits were 2 (SPT1) and 1 (SPT2) ppm BOD, with SEMs of 3.6% and 14.3% for the SPT1 and SPT2 sensors, respectively. Based on the results obtained in this study, it is concluded that SPT2 is the more suitable biocatalyst for pulp mill wastewater analysis. / While 18S rRNA gene sequence analyses, including BLAST homology searches, have suggested that isolate SPT1 is a close relative of Candida sojae (99.8% homology), no close matches have been found for isolate SPT2. The closest match for SPT2 was to Candida krusei (76.0% homology). Evidence from biochemical tests, fatty acid analysis, and 18S rRNA gene sequence analyses, indicates that isolate SPT2 is a novel yeast species.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.30759 |
| Date | January 2000 |
| Creators | Trosok, Steve Peter Matyas. |
| Contributors | Luong, John (advisor), Driscoll, Brian (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Natural Resource Sciences.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001764148, proquestno: MQ64470, Theses scanned by UMI/ProQuest. |
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