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Previous issue date: 2015-08-31 / Peripheral nerve injuries are a common clinical problem and the development
of new approaches to assist in its repair has been shown to be a growth area. The
use of microstructured channels has been investigated, in order to promote cell
growth guided by assisting in the damaged tissue recovery. Biocompatible and
biodegradable materials can be applied for the construction of these devices by
eliminating the need for a second procedure to remove the implant. This work
proposes the preparation of microstructured film along channels aligned with
polydimethylsiloxane (PDMS) and blends of polycaprolactone (PCL) and poly (lacticco-
glycolic acid) (PLGA) for potential application in peripheral nerve regeneration.
PDMS films were prepared by the method of mixing the solution with oligomers
based curing agent. Blends of PCL (Mn = 80.00) and PLGA (85:15) with different
ratios of PCL / PLGA (100/0, 90/10, 80/20 and 70/30) were prepared by the solvent
evaporation method. The mold used to produce the films consisted of channels with
different widths (5, 10, 15 and 20 ?m) and constant height (25 ?m). The materials
were characterized by Scanning Electron Microscopy (SEM), Infrared Spectroscopy
Fourier Transform (ATR / FT-IR), thermal gravimetric analysis (TGA), Atomic Force
Microscopy (AFM). To evaluate the degradation time of the films was carried out in
vitro degradation test of PCL blends and PCL / PLGA observing weight loss 0.4 to
9.9% until 90 days testing. It was found that the addition of the PLGA PCL is an
effective way to control the degradation rate of the blend and there is no significant
difference in the degradation of flat and microstructured films. / Les?es no nervo perif?rico s?o um problema cl?nico comum e a constru??o de
novos dispositivos para auxiliar no seu reparo tem se mostrado uma ?rea em
crescimento. A utiliza??o de condutos microestruturados vem sendo investigada,
com o objetivo de promover um crescimento celular guiado, auxiliando na
recupera??o do tecido lesado. Materiais biocompat?veis e biodegrad?veis podem ser
aplicados para a constru??o destes dispositivos eliminando a necessidade de um
segundo procedimento para remo??o do implante. Este trabalho prop?e a
prepara??o de filmes microestruturados com canais longitudinalmente alinhados de
polidimetilsiloxano (PDMS) e blendas de policaprolactona (PCL) e poli(?cido l?cticoco-
glic?lico) (PLGA) para potencial aplica??o em regenera??o de nervo perif?rico.
Filmes de PDMS foram preparados pelo m?todo de mistura da solu??o base de
olig?meros com agente de cura. Blendas de PCL (Mn = 80,00) e PLGA (85:15) com
diferentes raz?es de PCL/PLGA (100/0, 90/10, 80/20 e 70/30) foram preparadas pelo
m?todo de evapora??o do solvente. O molde utilizado para produzir os filmes era
constitu?do de canais com diferentes larguras (5, 10, 15 e 20 ?m) e altura constante
(25 ?m). Os materiais foram caracterizados por Microscopia Eletr?nica de Varredura
(MEV), Espectroscopia no Infravermelho por Transformada de Fourier (ATR/FT-IR),
An?lise termogravim?trica (TGA), Microscopia de For?a At?mica (AFM). Para
verificar o tempo de degrada??o dos filmes foi realizado ensaio de degrada??o in
vitro das blendas de PCL e PCL/PLGA, observando-se perda de massa de 0,4-9,9 %
at? o per?odo de 90 dias de ensaio. Constatou-se que a adi??o de PLGA ao PCL ?
uma forma efetiva de controlar a taxa de degrada??o das blendas e que n?o h?
diferen?a significativa entre a degrada??o dos filmes lisos e microestruturados.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/6538 |
| Date | 31 August 2015 |
| Creators | Garcez, Giovanna Ramos |
| Contributors | Basso, Nara Regina de Souza |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Engenharia e Tecnologia de Materiais, PUCRS, Brasil, Faculdade de Engenharia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -7432719344215120122, 600, 600, 600, -655770572761439785, 4518971056484826825 |
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