<p>Predmet doktorske disertacije je ispitivanje mogućnosti karakterizacija novih biorazgradivih kompozitnih filmova na<br />bazi biljnih proteina. Osnovno istraživanje se bazira na<br />ispitivanju mogućnosti dobijanja kompozitnih filmova na bazi<br />obnovljivih sirovina, vlažnim postupkom ("casting" metoda),<br />karakterizaciji dobijenih filmova i modifikaciji uslova dobijanja<br />radi poboljšanja osobina formiranog filma. Istraživanja se<br />zasnivaju na dobijanju filmova na bazi pogače uljane tikve<br />golice (Cucurbita pepo L. c. v. Olinka) (pumpkin oil cake –<br />PuOC), njenog proteinskog izolata i njihove kombinacije sa<br />drugim filmogenim polimerima (proteinima i polisaharidima).<br />Istraživanje podrazumeva procenu mogućnosti primene PuOC<br />radi delimične zamene opšte poznatih filmogenih materijala,<br />kao i produkciju filmova od PuOC i od proteinskog izolata<br />PuOC. Za produkciju filmova, od važnosti je ispitivanje<br />procesnih parametara (temperatura, pH, period denaturacije,<br />uslovi sušenja, itd.) i komponenti koje formiraju film (količina<br />polimera sa sposobnošću formiranja filma, količina i vrsta<br />plastifikatora, količina i vrsta agenasa za umrežavanje, itd.). S<br />obzirom na velik broj parametara koji utiču na formiranje filma,<br />kao i na osobine formiranih filmova, ispitano je međusobno<br />delovanja više faktora na mogućnost produkcije i osobine<br />dobijenog filma. Odabir i optimizacija procesnih parametara i modelovanje produkcije filmova izvedeno je implementiranjem<br />nove kompjuterske i analitičke metodologije. Osobine značajne<br />za dalju primenu dobijenih filmova podrazumevaju mehaničke<br />osobine (zateznu jačinu i izduženje pri kidanju), barijerne<br />(propustljivost gasova) i fizičko-hemijske karakteristike<br />(rastvorljivost, količinu rastvorljivih proteina, biološku<br />aktivnost u vidu antioksidantne aktivnosti), a ispitane su u cilju<br />deklarisanja potencijalne aplikacije filma. Dodatno, ispitana je<br />mogućnost produkcije kompozitnih filmova proteinski<br />izolat/hitozan, kao i primena enzimskog umrežavanja (crosslinking)<br />enzimom transglutaminaza (TGaza), radi dobijanja<br />filmova sa unapređenim karakteristikama. Istraživanja su<br />vođena i u smeru karakterizacije strukture nastalih filmova,<br />primenom tehnika elektron skenirajuće mikroskopije,<br />deferencijalne skening kalorimetrije, gasne hromatografije i<br />Furije transformišuće infracrvene spektrofotometrije.</p> / <p>The subject of the doctoral dissertation is to examine the possibility of<br />production and characterization of new biodegradable composite<br />films based on plant proteins. The research is based on an<br />examination of the possibility of obtaining composite films based on<br />renewable raw materials, using the casting method, on the<br />characterization of the obtained films and the requirement for the<br />modification to improve the properties of the formed film. The<br />research is aimed to evaluate the possibility of the production<br />the new biodegradable films based on hull-less pumpkin<br />(Cucurbita pepo L. c. v. Olinka) oil cake (pumpkin oil cake -<br />PuOC), its protein isolates and their combinations with other<br />filmogenous polymers (proteins and polysaccharides). The<br />study involves partial replacement of commonly well-known<br />filmogenous materials with PuOC, and production of films<br />based on the whole PuOC and the protein isolates from PuOC.<br />For film production, it is important to investigate the process<br />parameters (temperature, pH, denaturation period, drying conditions,<br />etc.), film-forming components (the amount of polymer with filmforming<br />ability, the amount and type of plasticizer, the amount and<br />type of cross-linking agents, etc..). As the large number of parameters<br />influence the film formation, as well as the properties of formed<br />films, the interaction of several factors which affect the possibility of<br />the production and properties of the obtained film, was examined.<br />Selection and optimization of process parameters and modeling of<br />film production will be carried out by implementing a new computer<br />and analytical methodology. Characteristics important for further<br />application of the obtained films include mechanical properties<br />(tensile strength and elongation at break), barrier (gas permeability)<br />and physical-chemical properties (solubility, the amount of soluble<br />proteins, biological activity in the form of antioxidant activity) and<br />were tested for the purpose of declaring potential application of the produced films. Additionaly, the possibility of production of composite films protein isolate/chitosan, and the application of enzymatic networks (cross-linking) by the enzyme<br />transglutaminase, in order to obtain films with improved<br />properties, was examined. Research was conducted in the<br />direction of the characterization of films formed by applying the<br />techniques of scanning electron microscopy, diferential<br />scanning calorimetry, gas chromatography and Fourier<br />transforming infrared spectrophotometry.</p>
| Identifer | oai:union.ndltd.org:uns.ac.rs/oai:CRISUNS:(BISIS)83221 |
| Date | 05 April 2013 |
| Creators | Popović Senka |
| Contributors | Peričin Draginja, Lazić Vera, Trivić Svetlana |
| Publisher | Univerzitet u Novom Sadu, Tehnološki fakultet Novi Sad, University of Novi Sad, Faculty of Technology at Novi Sad |
| Source Sets | University of Novi Sad |
| Language | Serbian |
| Detected Language | English |
| Type | PhD thesis |
| Format | application/pdf |
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