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Previous issue date: 2011-12-02 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / In the present study, six families of sulfated polysaccharides were obtained from seaweed Dictyopteris delicatula (Lamouroux, 1809) and their anticoagulant, antioxidant and antitumor activities were evaluated. All fractions showed anticoagulant activity on aPTT assay, but not on PT assay. Fractions also exhibited total antioxidant activity, superoxide radical scavenging capacity and ferric chelating property. Thus, six fractions (F0.5v, F0.7v, F1.0v, F1.3v, F1.5v e F2.0v) we obtained by proteolytic digestion, followed by acetone fractionation and molecular sieving on Sephadex G-100. Chemical analyses demonstrated that all polysaccharides contain heterofucans composed mainly of fucose, xylose, glucose, galactose, uronic acid, and sulfate. Any fractions changed the PT. However, all fractions were able on double the aPPT on a dose-dependent manner. The heterofucans F0.7v and F1.0v showed low anticoagulant activity while F1.5v presented the most prominent anticoagulant activity .When compared to Clexane?, a low molecular weight heparin, at same concentration F1.5v presented similar anticoagulant activity. The fucans F0.5v and F0.7v at 1.0 mg/mL showed high ferric chelating activity (~45%), whereas fucans F1.3v (0.5 mg/mL) showed considerable reducing power, about 53.2% of the activity of vitamin C. The fucan F1.5v presented the most prominent anticoagulant activity. The best antiproliferative activity was found with fucans F1.3v and F0.7v. However, F1.3v activity was much higher than F0.7v inhibiting almost 100% of HeLa cell proliferation. These fucans have been selected for further studies on structural characterization as well as in vivo experiments, which are already in progress / No presente estudo, seis popula??es de polissacar?deos sulfatados foram obtidas a partir da alga Dictyopteris delicatula (Lamouroux, 1809) e suas atividades anticoagulante, antioxidante e antitumoral avaliadas. Deste modo, as seis fra??es (F0.5v, F0.7v, F1.0v, F1.3v, F1.5v e F2.0v) foram obtidas por prote?lise seguida por fracionamento de acetona e gel filtra??o molecular Sephadex G-100. Todas as fra??es apresentaram atividade anticoagulante frente ao ensaio de aPTT, mas n?o sobre o ensaio de PT. As heterofucanas exibiram atividade antioxidante total, capacidade em sequestrar radicais super?xido e propriedade de quelar ferro. As an?lises qu?micas demonstraram que todos os polissacar?deos cont?m heterofucanas composta principalmente por fucose, xilose, glicose, galactose, ?cido ur?nico, e sulfato. Nenhuma das fra??es alterou o PT. Entretanto, todas as fra??es foram capazes de dobrar o aPTT de uma maneira dose dependente. As heterofucanas F0.7v e F1.0v demonstraram baixa atividade anticoagulante, enquanto a F1.5v apresentou a maior atividade anticoagulante e quando comparada com Clexane?, uma heparina de baixo peso molecular, em mesma concentra??o, apresentou antividade anticoagulante semelhante. As fucanas F0.5v e F0.7v a 1,0 mg/mL mostraram alta atividade de quela??o ferica (~ 45%), enquanto a fucana F1.3v (0,5 mg/mL) mostrou consider?vel potencial redutor, cerca de 53,2% da atividade da vitamina C. A melhor atividade antitumoral foi encontrada nas fucanas F1.3v e F0.7v. No entanto, a atividade F1.3v foi muito superior a F0,7v, quase 100% de inibi??o da prolifera??o de c?lulas HeLa (c?lula de c?ncer de colo de ?tero). Estas fucanas foram selecionadas para novos estudos sobre caracteriza??o estrutural, bem como em experimentos in vivo, que j? est?o em andamento / 2020-01-01
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/13382 |
| Date | 02 December 2011 |
| Creators | Magalhaes, Kaline Dantas |
| Contributors | CPF:76111830449, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4799567J8&dataRevisao=null, Filgueira, Luciana Guimar?es Alves, CPF:01843965496, http://lattes.cnpq.br/9951316929526841, Farias, Eduardo Henrique Cunha de, CPF:01164378473, http://lattes.cnpq.br/0933304924768138, Rocha, Hugo Alexandre de Oliveira |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Ci?ncias da Sa?de, UFRN, BR, Ci?ncias da Sa?de |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/embargoedAccess |
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