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Previous issue date: 2011-06-09 / Rice (Oryza sativa L.) is one of the major world crops, being widely consumed among people from five continents. In recent years research have increased in relation to the use of plant growth promoting diazotrophic bacteria (PGPB) as a possible solution to reduce the use of chemical nitrogen and phosphate fertilizers. Thus, this study aimed to evaluate the physiological potential and inorganic phosphate (IP) solubilization capacity of different diazotrophic strains isolated from rice plants grown in different regions of Brazil as well as to evaluate their role in the development of rice plants. Firstly, the culture media employed to test the phosphate solubilization in petri dishes were standardized. In this study 49 strains were tested for their capability of solibilizing tricalcium phosphate in NBRIP and GL solid media. Afterwards, the soluble P, pH, population and the index of solubilization (IS) were quantified for 7 IP solubilizing strains grown in the NBRIP liquid medium. The IP solubilizing isolates were also tested for the production of indolic compounds (IAA) and the nitrogen fixation capacity through the acetylene reduction activity (ARA). The isolates were taxonomical identified through the amplification and partial sequencing of 16S RNAr and nifH genes. Three of these strains and the controls H. seropedicae ZAE94, G diazotrophicus PAL5 and A.chroochoccum AC1 were tested in greenhouse conditions in association with rice plants. All strains that solubilized IP in NBRIP liquid medium decreased the medium pH while the bacterial population remained around 108 cfu ml-1 for up to 15 days. All strains were capable to produce indole compounds and reduced acetylene. The partial analysis of 16S RNAr and nifH gene indicated the identification of the species Herbaspirillum seropedicae, Burkholderia vietnamiensis and B. kururiensis species. All strains showed an increase in the range of 33 and 47% in grain yield when tricalcium phosphate (PTC) was applied and between 18 and 44% when PSS (simple superphosphate) was used. In general, all of the strains stimulated an increase of total P in the grain and a greater harvest index. The use of these strains as bioinculant is promising and therefore it is necessary to test in the field conditions / O arroz (Oryza sativa L.) ? uma das principais culturas cultivadas, sendo amplamente consumida nos cinco continentes. Nos ?ltimos anos as pesquisas t?m aumentado em rela??o ao uso de bact?rias diazotr?ficas e promotoras de crescimento vegetal (BPCV), como uma poss?vel solu??o para diminuir o uso de fertilizantes qu?micos nitrogenados e fosfatados. Desta forma, este trabalho teve por objetivo avaliar o potencial fisiol?gico e a capacidade de solubiliza??o de fosfato inorg?nico (PI) de diferentes estirpes de bact?rias diazotr?ficas oriundas de plantas de arroz cultivadas em diferentes regi?es do Brasil e seu papel no desenvolvimento de plantas de arroz. Primeiramente foi padronizado o meio de cultura a ser empregado nos ensaios de solubiliza??o de fosfato inorg?nico em placas de petri. Neste estudo foram testadas 49 estirpes em quanto sua capacidade de solubilizar fosfato tric?lcico (PTC) nos meios s?lidos NBRIP e GL. Em seguida foi quantificado o P sol?vel, pH, a popula??o de bact?rias e ?ndice de solubiliza??o (IS) das 7 estirpes solubilizadoras de PI em meio l?quido NBRIP. As estirpes solubilizadoras de PI tamb?m foram testadas quanto ? produ??o de compostos ind?licos (AIA) e capacidade de fixar nitrog?nio atrav?s da atividade de redu??o e acetileno (ARA). As estirpes foram identificadas atrav?s da amplifica??o e do sequenciamento parcial dos genes 16S RNAr e gene nifH. Tr?s destas estirpes foram testadas em condi??es de casa de vegeta??o junto com os controles Herbaspirillum seropedicae BR11417 (ZAE94), Gluconacetobacter diazotophicus BR11281 (PAL5) e Azotobacter Chroochoccum AC1 quanto ? capacidade de aumentar o desenvolvimento e produ??o de plantas de arroz. Todas as estirpes que solubilizaram PI no meio de cultura NBRIP l?quido influenciaram na diminui??o do pH do meio enquanto que a popula??o se manteve em torno de 108 ufc.ml-1 por at? 15 dias. Todas as estirpes apresentaram capacidade de produzir compostos ind?licos e foram capazes de reduzir acetileno. A an?lise parcial do gene 16S RNAr e do gene nifH das sete estirpes possibilitou a identifica??o das esp?cies Herbaspirillum seropedicae, Burkholderia vietnamiensis e B. kururiensis. Todas as estirpes promoveram um aumento na produ??o de massa seca nos gr?os entre 33 e 47% quando foi utilizado o PTC e de 18 a 44% quando foi utilizado o fosfato super simples (PSS). Em geral, todas as estirpes estimularam um maior acumulo de N e P total no gr?o e um maior ?ndice de colheita. O uso dessas estirpes como bioinoculante ? bastante promissor e portanto torna-se necess?rio test?-las em condi??es de campo.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:jspui/1166 |
| Date | 09 June 2011 |
| Creators | Bonilla, German Andres Estrada |
| Contributors | Baldani, Jos? Ivo, Baldani, Vera Lucia Divan |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Fitotecnia, UFRRJ, Brasil, Instituto de Agronomia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 6. REFER?NCIAS BIBLIOGR?FICAS ALIKHANI, H.A.; SALEH-RASTIN, N.; ANTOUN, H. Phosphate solubilization activity of rhizobia native to Iranian soils. Plant and Soil, v. 287, p.35?41, 2006. ALVES, J. R.; BODDEY, R. M.; URQUIAGA S. The success of BNF in soybean in Brazil. Plant and soil, v. 252, p. 1-9, 2003. ANTOUN, H. A.; BEAUCHAMP, C. J.; GOUSSARD, N.; CHABOT, R.; LALANDE, R. Potential of Rhizobium and Bradyrhizobium species as plant growth promoting rhizobacteria on non-legumes: effect on radishes (Raphanus sativus L.). Plant and Soil, v.204, p.57?67, 1998. ARA?JO, W. L.; MARCON, J.; MACCHERONI JUNIOR, W.; VAN ELSAS, J. D.; VAN VUURDE, J. W. L.; AZEVEDO, J. L. Diversity of endophytic bacterial populations and their interaction with Xilella fastidiosa in citrus plants. Applied and Environmental of Microbiology, v.68, p.4906-4914, 2002. ARA?JO, A. S. Caracteriza??o e uso de bacterias diazotr?ficas isoladas de diferentes cultivares de arroz origin?rias do estado do maranh?o. Seropedica, Universidade Federal Rural do Rio de Janeiro, 2008. 88p. Tese (Doutorado em Fitotecnia, Agroecologia). Instituto de Agronomia, Departamento de Fitotecnia. Tese de doutorado. ASGHAR, H.N.; ZAHIR, Z.A.; ARSHAD, M.; KHALIQ, A. Relationship between in vitro production of auxins by rhizobacteria and their growth-promoting activities in Brassica juncea L. Biology and Fertility of Soils, v.35, p.231-237, 2002. AZAMBUJA, I.H.V., VERNETTI Jr., F.J., MAGALH?ES Jr., A. M. Arroz irrigado no sul do Brasil. Aspectos socioecon?micos da produ??o do arroz. Embrapa Informa??o Tecnol?gica, p.23-44, 2004. BACA, B.; SOTO, L.; PARDO, M. Fijaci?n biol?gica de nitr?geno. Elementos, v.1, p. 39?49, 2000. BACILO-JIMENEZ, M.; AGUILAR-FLORES, S.; VENTURA-ZAPATA, E.; PEREZ- CAMPOS, E.; BOUQUELET, S.; ZENTENO, E. Chemical characterization of root exudates from rice (Oryza sativa) and their effects on the chemotactic response of endophytic bacteria. Plant and Soil, v.249, p.271?277, 2003. BALANDREAU, J. Microbiology of the association. Canadian Journal of Microbiology, v.29, p.851?859, 1983. BALDANI, J.I.; BALDANI, V.L.D.; SELDIN, L.; D?BEREINER, J. Characterization of Herbaspirillum seropedicae gen. nov., sp. nov., a root-associated nitrogen-fixing bacterium. International Journal of Systematic Bacteriology, v.36, p.86?93, 1986. BALDANI, V. L. D. Efeito da Inocula??o de Herbaspirillum spp.. no processo de coloniza??o e infec??o de plantas de arroz, e ocorr?ncia e caracteriza??o parcial de 49 uma nova bact?ria diazotr?fica. 1996. 238 p. Tese (Doutorado em Ci?ncia do Solo) - Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ. BALDANI, V.L.D.; BALDANI, J.I.; D?BEREINER, J. Inoculation of rice plants with the endophytic diazotrophs Herbaspirillum seropedicae and Burkholderia spp. Biology and Fertility of Soils, v.30, p.485-491, 2000. BARTEL, B. Auxin biosynthesis. Annual Review in Plant Physiology and PLANT Molecular Biology, v.48, p.51?66, 1997. BASHAN, Y. Inoculants of plant growth-promoting bacteria for use in agriculture. Biotechnology Advances, v.16, p.729?77, 1998. BASHAN, Y.; HOLGUIN, G.; DE-BASHAN, L.E. Azospirillum-plant relationships: agricultural, physiological, molecular and environmental advances (1997?2003). Canadian Journal Microbiology, v.50, p.521?5, 2004. BASTIAN, F.; COHEN, A.; PICCOLI, P.; LUNA, V.; BARALDI, R; BOTTINI, R. Production of indole-3-acetic acid and giberellins A(1) and A(3) by Gluconacetobacter diazotrophicus and Herbaspirillum seropedicae in chemically-defined culture media. Plant Growth Regulation, v. 24, n.1, p.7-11, 1998. BELIMOV, A.A.; DODD, I.C.; HONTZEAS, N.; THEOBALD ,J.C.; SAFRONOVA, V.I.; DAVIES, W.J. Rhizosphere bacteria containing ACC deaminase increase yield of plants grown in drying soil via both local and systemic hormone signalling. New Phytologist, v.181, p.413?423, 2009. BIELESKI, R.L. Phosphate pools, phosphate transport and phosphate availability. Annual Review of Plant Physiology, v.24, p.225?252, 1973. BLOEMBERG, G. V.; LUGTENBERG, B. J. J. Molecular basis of plant growth promotion and biocontrole by rhizobacteria. Current Opinion in Plant Biology, v.4, p.343-350, 2001. BODDEY, R. M.; OLIVEIRA, O. C. D.; URQUIAGA, S.; REIS, V. M.; OLIVARES, F. L.; D., BALDANI, V. L. D.; D?BEREINER, J. Biological nitrogen fixation associated with sugarcane and rice: contributions and prospects for improvement. Plant and Soil, v.174, p.195?209, 1995. BONILLA, R.; NOVO, R.; VENEGAS, N.; GALVIS, A.M.; MART?NEZ, M.M.; PARRA, D.; VANEGAS, O. Generaci?n de Tecnolog?as para la utilizaci?n de la fijaci?n no simbi?tica de nitr?geno como alternativa de la fertilizaci?n. Programa Regional de Investigaci?n Agr?cola CORPOICA-Regional v.3, p.12-30, 2000. BRADFORD, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Analytical Biochemistry, v.72, p.248-254, 1976. BRADY,N.; WEIL, R. 2004. Elements of the nature and properties of soils. Segunda edi??o. Editorial Prentice Hall. 606 p. 50 BRANDL, M.T.; LINDOW, S. E. Cloning and characterization of a locus encoding an indolepyruvate decarboxylase involved in indole-3-acetic acid synthesis in Erwinia herbicola. Applied and Environmental Microbiology, v.62, p.4121-4128, 1996. BRASIL, M. Ocorr?ncia e Diversidade Gen?tica de Bact?rias Diazotr?ficas Endof?ticas em Variedades de Arroz RJ. 2005. 105 p. Tese (Doutorado em Fitotecnia) Universidade Federal Rural de Rio de Janeiro, Serop?dica, RJ. BROWN, M. E.; BURLINGHAM, S. W. Production of plant growth substances by Azotobacter chroococcum. Journal of General Microbiology, v.53, p.135-144, 1968. CABALLERO, T.; CAMELO, M.; BONILLA, R.; MART?NEZ, M. Determinaci?n de Actividad Fosfatosolubilizadora por bacterias aisladas a partir de suelos algodoneros en los Departamentos del Cesar y Meta. Suelos Ecuatoriales, 37(1): 94-100, 2007. CHEN, Y.; REKHA, P.; ARUN, A.; SHEN, F.; LAI, W.; YOUNG C. Phosphate solubilizing bacteria from subtropical soil and their tricalcicum phosphate solubilizing abilities. Applied Soil ecology, v.34, p33-41, 2006. CHO, J. C.; VERGIN, K. L.; MORRIS, R. M.; GIOVANNONI, S. J. Lentisphaera araneosa gen. nov., sp. nov., a transparent exopolymer producing marine bacterium, and the description of a novel bacterial phylum, Lentisphaerae. Environmental Microbiology, v.6, p.611?621, 2004. CHOULDHURY, A. T. M. A.; KENNEDY, I. R. Prospects and potentials for systems of biological nitrogen fixation in sustainable rice production. Biology and Fertility of Soils, v.39, n 4, p.219?227, 2004. CLARK, E.; MANULIS, S.; OPHIR, Y.; BARASH, I.; GAFNI, Y. Cloning and characterization of iaaM and iaaH from Erwinia herbicola pathovar gypsophilae. Phytopathology, v.83, p.234?240, 1993. COCKING, E.C. Concerted action for cereal and other non-legume crop nitrogen fixation, enhanced growth and yield. in: KENNEDY, I.R.; CHOUDHURY, A.T.M.A. (Eds.). Biofertilizers in Action, Rural Industries Research and Development Corporation, Canberra, p. 1?3, 2002. COMPANHIA NACIONAL DE ABASTECIMENTO (CONAB). Acompanhamento de safra brasileira: gr?os, quinto levantamento, fevereiro 2011. Dispon?vel em http://www.conab.gov.br/conabweb/download/safra/4graos_07.01.10.pdf. Acesso em: 15 de fevereiro de 2011. CONG, P.T.; DUNGA, T.D.; HIEN, T.M.; HIEN, N.T.; CHOUDHURY, A.T.M.A.; KECSK?S, M.L.; KENNEDY, I.R. Inoculant plant growthpromoting microorganisms enhance utilization of urea-N and grain yield of paddy rice in southern Vietnam. European ournal of soil biology, v.45, p.52?61, 2009. 51 COSTACURTA, A.; KEIJERS, V.; VANDERLEYDEN, J. Molecular cloning and sequence analysis of an Azospirillum brasilense indole-3-pyruvate decarboxylase gene. Molecular and General Genetics, v.243, p.463?472, 1994 COSTACURTA, A.; VANDERLEYDEN, J. Synthesis of phytohormones by plant-associated bacteria. Critical Reviews in Microbiology, v.21, p.1-18, 1995. CRESPO, J. M.; BOIARDI, J. L.; LUNA, M. F. Mineral phosphate solubilization activity of Gluconacetobacter diazotrophicus under P-limitation and plant root environment. Agricultural Sciences, v.2, p.16-22, 2011. DARDANELLI, M.S.; CARLETTI, S.M.; PAULUCCI, N.S.; MEDEOT, D.B.; C?CERES, E.A.R.; VITA, F.A.; BUENO, M.; FUMERO, M.V.; GARCIA, M.B. Benefits of plant growth-promoting rhizobacteria and rhizobia in agriculture. In: MAHESHWARI, D.K. (Ed.). Plant Growth and Health Promoting Bacteria. Springer-Verlag, Berlin Heidelberg, p.1-20, 2010. DE SALAMONE, I.; HYNES, R.; NELSON, L. Cytokinin production by plant growth promoting rhizobacteria and selected mutants. Canadian Journal of Microbiology, v.47, p.404-411, 2001. DENNIS, N. Reinventing rice to feed the world. SCIENCE, v.321, p.330-333, 2008. DEUBEL, A.; MERBACH, W. Influence of microorganisms on phosphorus bioavailability in soils. in: BUSCOT, F. VARMA, A. (Eds.). Microorganisms in soils: roles in genesis and functions. Springer, Berlin, p. 177?191, 2005. DI, H.J.; CAMERON, K.C. Nitrate leaching in temperate agroecosystems: sources, factors and mitigating strategies. Nutrient Cycling in Agroecosystems, v.64 ,n.3, p.237?256, 2002. DIDONET, A. D.; MARTIN-DIDONET, C. C. G.; GOMES, G. F. Avalia??o de linhagens de arroz de terras altas inoculadas com Azospirillum lipoferum Sp59b e Azospirillum brasilense Sp24. Comunicado T?cnico EMBRAPA, n. 69, dez. 2003. DIMKPA, C.; WEINAND, T.; ASCH, F. Plant?rhizobacteria interactions alleviate abiotic stress conditions. Plant, Cell e Environment, v. 32, n.12, p.1682-1694, 2009. D?BEREINER, J.; BALDANI, V.L.D.; BALDANI, J.I. Como isolar e identificar bact?rias diazotr?ficas de plantas n?o leguminosas. Bras?lia: Embrapa- SPI, Itagua?, RJ. Embrapa Agrobiologia, 60p, 1995. DOBBELAERE, S.; CROONENBORGHS, A.; THYS, A.; PTACEK, D.; VANDERLEYDEN, J.; DUTTO, P.; LABANDERA-GONZALEZ, C.; CABALLERO-MELLADO, J.; ANGUIRRE, J.F.; KAPULNIK, Y.; BRENER, S.; BURDMAN, S.; KADOURI, D.; SARIG, S.; OKON, Y. Response of agronomically important crops to inoculation with Azospirillum. Australian Journal Plant Physiology, v.28, p.871?879, 2001. 52 DOBBELAERE S, V.; ANDELEYDEN, J.; OKON,Y. Plant growth-promoting effects of diazotrophs in the rhizosphere. Critical Reviews in Plant Sciences, Boca Raton, v.22: p.107-149, 2003. DODD, I.C. Root-to-shoot signalling: assessing the roles of ??up?? in the up and down world of long-distance signalling in planta. Plant and Soil, v.274, p.251?270, 2005. DODD, I.C.; ZINOVKINA, N.Y.; SAFRONOVA, V.I.; BELIMOV, A.A. Rhizobacterial mediation of plant hormone status. Annals of Applied Biology, v. 157, n.3, p.361-379, 2010. EWING, B.; HILLIER, L.; WENDL, M.C.; GREEN, P. Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome Research, v.8, p;175-185,1998. EWING, B. GREEN, P. Base-calling of automated sequencer traces using phred. II. Error probabilities. Genome Research, v. 8, p.186-194, 1998. EGEBO, L.A.; NIELSEN, S.V.S.; JOCHIMSEN, B.U. Oxygen-dependent catabolism of indole-3-acetic acid in Bradyrhizobium japonicum. Journal of Bacteriology, v.173, p. 4897?4901, 1991. ELIASSON, L.; BERTEL, G.; BOLANDER, E. Inhibitory action of auxin on root elongation not mediated by ethylene. Plant Physiology, v.91, p.310?314, 1989. ESTRADA, G. A.; BONILLA, R. R.; DIVAN BALDANI, V. L. Efecto de diferentes temperaturas de almacenamiento sobre la calidad de bioinoculantes turbosos. Revista Corpoica - Ciencia y Tecnologia Agropecuarias, v.10, n.2, p. 205-213, 2009. EUCLYDES, R. F. Manual de utiliza??o do programa SAEG (Sistema de An?lise Estat?stica e Gen?tica). Vi?osa, MG: Universidade Federal de Vi?osa, 1983. 59p. EUROPEAN FERTILIZER MANUFACTURER ASSOCIATION (EFMA). Phosphorus Essential Element for Food Production. Dispon?vel em: http://www.efma.org/documents/file/publications/Understanding%20Phosphorus%20and%20its%20Use%20in%20Agriculture%20(2000).pdf. Acesso em 10 de janeiro de 2011. FAO. FAOSTAT Database. Dispon?vel em: http://faostat.fao.org/site/339/default.aspx Acesso em: 15 de janeiro de 2011. FENGLEROWA, W. Simple method for counting Azotobacter in soil samples. Acta. Microbiologica Polonica, v.14, n.21, p.203, 1965. FELSENSTEIN, J. Confidence limits on phylogenies: An approach using the bootstrap. Evolution v.39, p.783-791, 1985. FERREIRA, D. F. An?lises estat?sticas por meio do Sisvar para Windows vers?o 5.0. In: 45a Reuni?o Anual da Regi?o Brasileira da Sociedade internacional de Biometria. UFSCar, S?o Carlos, SP, 2003. p.255-258. 53 FERREIRA, J.S.; SABINO, D.C.C.; GUIMAR?ES, S.L.; BALDANI, J.I.; BALDANI, V.L.D. Sele??o de ve?culos para o preparo de inoculante com bact?rias diazotr?ficas para arroz inundado. Revista Agronomia, v.37, n.02, p.6-12, 2003. FERREIRA, J.S. Sele??o e avalia??o de ve?culos para inocula??o de bact?rias diazotr?ficas na cultura do arroz inundado. 2004. 44p. Disserta??o (Mestrado em Agronomia ? Ci?ncia do solo) - Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ. FERREIRA, J.S. Qualidade de inoculante, inocula??o e reinocula??o de Herbaspirillum seropedicae em duas variedades de arroz irrigado. 2008. 83p. Tese (Doutorado em Agronomia ? Ci?ncia do solo) - Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ. FERREIRA, J.S.; BALDANI, J.I.; BALDANI, V.L.D. Sele??o de inoculantes ? base de turfa contendo bact?rias diazotr?ficas em duas variedades de arroz. Acta Scientiarum. Agronomy, v.32, n.1, p.179-185, 2010. FRANCHE, C.; LINDSTR?M, K.; ELMERICH, C. Nitrogen-fixing bacteria associated with leguminous and non-leguminous plants. Plant and Soil. v.321, p.35?59, 2009. GANS, J.; WOLINSKY, M.; DUNBAR, J. Computation improvements reveal great bacterial diversity and high metal toxicity in soil. Science, v.309, p.1387?139, 2005. GILLER, K. (2001): Nitrogen fixation in tropical cropping systems. CABI Publishing, Wallingford, UK, 423p. GILLIS, M.; TRAN VAN, V.; BARDIN, R.; GOOR, M.; HEBBAR, P.; WILLEMS, A.; SEGERS,P.; KERSTERS, K.; HEULIN, T.; FERNANDEZ, M.P. Poly phasic taxonomy in the genus Burkholderia leading to a amended description of the genus and proposition o Burkholderia vietmaniensis sp. nov. for N2-fixing isolates from rice in Vietnam. International Journal of Systematic Bacteriology, v.45, p.274?289, 1995. GLICK, B.R.; CHENG, Z.; CZARNY, J.; DUAN, J. Promotion of plant growth by ACC deaminase-producing soil bacteria. European Journal of Plant Pathology, v.119, p.329?339, 2007. GLICKMANN, E.; GARDAN, L.; JACQUET, S.; HUSSAIN, S.; ELASRI, M.; PETIT, A.; DESSAUX, Y. Auxin production is a common feature of most pathovars of Pseudomonas syringae. Molecular Plant?Microbe Interaction, v.11, p.156?162, 1998. GOLDSTEIN, A. H. Bacterial solubilization of mineral phosphates: historical perspectives and future prospects. American Journal of Alternative Agriculture, v.1, p.51?57, 1986. GOLDSTEIN, A.H. Recent progress in understanding the molecular genetics and biochemistry of calcium phosphate solubilization by gram negative bacteria. Biology, Agriculture and Horticulture, v.12, p.185?193, 1995. GORDON, S.A.; WEBER,P.R. Colorimetric estimation of indol acetic acid. Plant Physiology, v.26: p.192-195, 1950. 54 GORDON, D.; ABAJIAN, C.; GREEN, P. Consed: A Graphical Tool for Sequence Finishing. Genome Research, v.8, p.195-202, 1998. GOVINDARAJAN, M.; BALANDREAU, J.; KWON, S.; WEON, H.; LAKSHMINARASIMHAN, C. Effects of the Inoculation of Burkholderia vietnamiensis and Related Endophytic Diazotrophic Bacteria on Grain Yield of Rice. Microbial Ecology, v.55, p.21?37, 2008. GRAY, E.; SMITH, D.L. Intracellular and extracellular PGPR: Commonalities and distinctions, in the plant bacterium signaling process. Soil biology and soil biochemistry, v.37, p.395?412, 2005. GUIMAR?ES, C.M.;DOS SANTOS, A.B.; J?NIOR, A.M.M.; STONE, L.F. Sistemas de cultivo. In: SANTOS, A.B.; STONE, L.F.; VIEIRA, N.R.R. (Eds.). A cultura do arroz no Brasil. Santo Ant?nio de Goi?s: Embrapa-CNPAF, p.53-96, 1998. GUIMAR?ES, S. L. sele??o de estirpes de bact?rias diazotr?ficas endof?ticas para inocula??o em tr?s cultivares de arroz inundado. 2001. 52p. Disserta??o (mestrado em Agronomia ? Ci?ncia do solo) - Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ. GUIMAR?ES, S.L., BALDANI, J.I., BALDANI, V.L.D. Efeito da inocula??o de bact?rias diazotr?ficas endof?ticas em arroz de sequeiro. Revista Agronomia, v37, n.2, p.25-30, 2003. GUIMAR?ES, S. L. Aplica??o de inoculante turfoso com bact?rias diazotr?ficas e molibd?nio em cultivares de arroz adubadas com nitrog?nio mineral. 2006. 52p Tese (Doutorado em Agronomia - Fitotecnia) - Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ. GUIMAR?ES, S. L.; BALDANI, J. I.; BALDANI, V. L. D.; JACOB-NETO, J. Adi??o de molibd?nio ao inoculante turfoso com bact?rias diazotr?ficas usado em duas cultivares de arroz irrigado. Pesquisa Agropecu?ria Brasileira, v. 42, n. 3, p. 393-398, 2007. GUIMAR?ES, A. P. Bases bioqu?micas do fracionamento isot?pico de 15n na fixa??o biol?gica de nitrog?nio e da efici?ncia energ?tica do processo em diferentes estirpes de bact?rias diazotr?ficas. 79p. Tese ( Doutorado em Produ??o Vegetal). Universidade Estadual do Norte Fluminense, Campus, RJ, 2010. GYANESHWAR, P.; PAREKH, L. J.; ARCHANA, G.; PODLE, P. S.; COLLINS, M. D.; HUTSON R. A.; NARESH. K. G. Involvement of a phosphate starvation inducible glucose dehydrogenase in soil phosphate solubilization by Enterobacter asburiae. FEMS Microbiology Letter, v.171, p.223-229, 1999. GYANESHWAR, P.; KUMAR, G. N.; PAREKH, L. J.; POOLE, P. S. Role of soil microorganisms in improving P nutrition of plants. Plant and Soil, v.245, p.83-93, 2002. HALDER, A.; CHAKRABARTTY, P. Solubilization of inorganic phosphates by Rhizobium. Folia Microbiologica, v.38, p.325-330, 1993. 55 HAMEEDA, B.; HARINI, G.; RUPELA, O.P.; WANI, S.P.; REDDY, G. Growth promotion of maize by phosphate-solubilizing bacteria isolated from composts and macrofauna. Microbiological Research, v.163, p.234?24, 2008. HARTMANN, A.; SINGH, M.; KLINGMULLER ,W. Isolation and characterization of Azospirillum mutants excreting high amounts of indoleacetic acid. Canadian journal microbiology .v.29, p.916?923, 1983. HUANG, X.; MADAN, A. CAP3: A DNA sequence assembly program. Genome Research, v.9, p.868-877, 1999. HUNGRIA, M.; LOUREIRO, M.F.; MENDES, I.C.; CAMPO, R.J.; GRAHAM P.H. Inoculant preparation, production and application, In: WERNER, D.; NEWTON W.E. (Eds.). Nitrogen Fixation in Agriculture, Forestry, Ecology and the Environment, Springer, Dordrecht, The Netherlands, p. 223?254, 2005, IGUAL JM, RODRIGUEZ-BARRUECO C. Fertilizers, food and environment. In: VELAZQUEZ, E.; RODRIGUEZBARRUECO, C. (Eds.). First International Meeting on Microbial Phosphate Solubilization. Dordrecht: Developments in Plant and Soil Science, v.102, p.199?202, 2007. INTORNE, A.C.; DE OLIVEIRA, M.V.; LIMA, M.L.; DA SILVA, J.F.; OLIVARES, F.L.; DE SOUZA FILHO, G.A. Identifcation and characterization of Gluconacetobacter diazotrophicus mutants defective in the solubilization of phosphorus and zinc. Archives of Microbiology, v.191, p.477-483, 2009. Instituto Brasileiro de Geografia e Estat?stica (IBGE). Levantamento Sistem?tico da Produ??o Agr?cola. Dispon?vel em http://www.ibge.gov.br/home/estatistica/indicadores/agropecuaria/lspa/lspa_200912comentarios.pdf. Acesso em: 20 de janeiro de 2010. JAIN, D.K.; PATRIQUIN, D.G. Characterization of a substance produced by Azospirillum which causes branching on wheat root hairs. Canadian Journal of Microbiology, v. 31, p. 206-210, 1985. JAKOBSEN, I.; LEGGETT, M.E.; RICHARDSON, A.E. Rhizosphere microorganisms and plant phosphorus uptake. In: SIMS, J.T.; SHARPLEY, A.N. (Eds.). Phosphorus, Agriculture and the Environment. American Society for Agronomy, Madison, p.437?494, 2005. JONES, R. D. Phosphorus cycle. In: LEDERBERG, J (ed.). Encyclopedia of Microbiology, Academic, San Diego, v.3, 2. edn. . p. 614?617, 2000. KENNEDY, G.; BURLINGAME, B. Analisys of food composition data of rice from a plant genetic resource perspective. Food Chemistry, v.80, n.4, p.589-596, 2003. KHALID, A.; ARSHAD, M. e ZAHIR, Z. A. Growth and yield response of wheat to inoculation with auxin producing PGPR. Pakistan Journal of Botany, v.35, p.483-498, 2004. 56 KHALIQ, A.; ARSHAD, M.; ZAHIR, Z.A. Screening plant growth-promoting rhizobacteria for improving growth and yield of wheat. Journal of Applied Microbiology, v. 96, p. 473 ? 480, 2004. KIMURA, M. A. simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, v.16, p.111-120, 1980. KOBAYASHI, M.; IZUI, H.; NAGASAWA, T.; YAMADA H. Nitrilase in biosynthesis of the plant hormone indole-3-acetic acid from indole-3-acetonitrile ? cloning of the Alcaligenes gene and sitedirected mutagenesis of cysteine residues. Proceedings of the National Academy of Sciences of the United States of America, v.90, p.247?251, 1993. KOBAYASHI, M.; SUZUKI, T.; FUJITA, T.; MASUDA, M.; SHIMIZU, S. Occurrence of enzymes involved in biosynthesis of indole-3-acetic acid from indole-3-acetonitrile in plant-associated bacteria, Agrobacterium and Rhizobium. Proceedings of the National Academy of Sciences of the United States of America. v.92, p.714?718 1995. KOGA, J.; ADACHI T.; HIDAKA H. Molecular cloning of the gene for indolepyruvate decarboxylase from Enterobacter cloacae. Molecular Genetics and Genomics, v.226, p.10?16, 1991. KPOMBLEKOU, K.; TABATABAI, M. Effects of organic acids on release of phosphorous from rock phosphate. Soil Sciences, v.158, p.442?453, 1994. KUCEY, R. M. N.; JANZEN H. H.; LEGGETT M. E. Microbially mediated increases in plant-available phosphorus. Advances in Agronomy, v.42, p.199-227, 1989. KUMAR, V.; NARULA, N. Solubilization of inorganic phosphates and growth emergent of wheat as affected by Azotobacter chrococum mutants. Biology and Fertility of Soils, v.28, p.301-305, 1999. KUMAR, V.; BEHL, R.K.; NARULA, N. Establishment of phosphate solubilizing strains of Azotobacter chroococcum in the rhizosphere and their effect on wheat cultivars under greenhouse conditions, Microbiological Research, v.156, p.87?93, 2001. KUNDU, B.; GAUR, A. Establishment of nitrogen fixing and phosphate solubilizing bacteria in rhizosphere and their effect on yield and nutrient uptake of wheat crop. Plant and Soil, v.57, p.223-230, 1980. KUNDU, D.K., LADHA, J.K. Engancing soil nitrogen use and biological nitrogen fixation em wetland rice. Experimental Agriculture, v. 31, n.3, p.261-278, 1995. LADHA, J.; REDDY, P. Nitrogen fixation in rice systems: state of knowledge and future prospects. Plant and Soil, v.252, p.151?167, 2003. LAKSHMINARAYANA, K.; NARULA, N.; HOODA, I. S.; FARODA, A. S. Nitrogen economy in wheat (Triticum aestivum L.) through use of Azotobacter chroococcum. Indian Journal of Agricultural Science, v.62, p.75?76, 1992. 57 LAMBRECHT, M.; OKON, Y.; VANDE BROEK, A.; VANDERELEYDEN, J. Indole-3-acetic acid: a reciprocal signalling molecule in bacteria-plant interactions. Trends in Microbiology, v.8, p.298-300, 2000. LATA, H.; LI, X.C.; SILVA, B. Identification of IAA producing endophytic bacteria from micropropagated echinacea plants using 16S rRNA sequencing. Plant Cell Tissue Organ Cult, v.85, p.353?359, 2006. LEAUNGVUTIVIROJ, C.; RUANGPHISARN, P.; HANSANIMITKUL, P.; SHINKAWA, H.; SASAKI, K. Development of a new biofertilizer with a high capacity for N2 fixation, phosphate and potassium solubilization and auxin production. Bioscience, Biotechnology, and Biochemistry. v.74, n.5, p.1098-1101, 2010. LEIGH,G J. Dinitrogen chemistry. In: LEIGH, G.L., (ed.). Nitrogen Fixation at the Millenium. Elsevier, Amsterdam, the Netherlands, p.299-327, 2002. LEVEAU, J.H.J.; LINDOW, S.E. Utilization of the plant hormone indole-3-acetic acid for growth by Pseudomonas putida strain 1290. Applied and Environmental Microbiology, v.71, p.2365?2371, 2005. LEVEAU, J.H.J.; GERARDS, S. Discovery of a bacterial gene cluster for catabolism of the plant hormone indole 3-acetic acid. FEMS Microbiology Ecology, v.65, p.238?250, 2008. LIN, T. F.; HUANG, H. I.; SHEN, F. T.; YOUNG, C. C. The protons of gluconic acid are the major factor responsible for the dissolution of tricalcium phosphate by Burkholderia cepacia CC-Al74. Bioresource Technology, v.97, p.957?960, 2006. LODEWYCKX, C. Endophytic bacteria and their potential applications. Critical Reviews in Plant Sciences, v.21, p.583-606, 2002. LOPEZ, M.; ESTRADA, G. A.; CAMELO, M.; BALDANI, V.L.; BONILLA, R.R.B. Determination of physiological potential in phosphate solubilizing and mineralizing by nitrogen fixing bacteria. In: 12th International symposium on biological nitrogen fixation with non-legumes, B?zios, 2010. LUCY, M.; REED, E.; GLICK, B. Applications of free living plant growth-promoting rhizobacteria. Antonie Van Leeuwenhoek, v.86, n.1, p.1-25, 2004 LUGTENBERG, B.; KAMILOVA, F. Plant-growth-promoting rhizobacteria. Annual Review of Microbiology, v.63, p.541-556, 2009. MAGALH?ES, F.M.; BALDANI, J.I.; SOUTO, S.M.; KUYKENDALL, J.R.; D?BEREINER, J. A new acid-tolerant Azospirillum species. Academia Brasileira de Ci?ncias, v.55, p.417?430, 1983. MAGALH?ES, C. L.; DE SOUZA, E. M.; WEBER, O. B.; BALDANI, J.I.; D?BEREINER, J.; PEDROSA F.O. 16S ribosomal DNA characterization of nitrogen-fixing bacteria isolated from banana (Musa spp.) and pineapple (Ananas comosus (L.) Merril). Applied and Environmental Microbiology, v.67, p.2375-2379, 2001. 58 MAHESHKUMAR, K.S.; KRISHNARAJ, P.U; ALAGWADI, A.R. Mineral solubilising activity of Acetobacter diazotrophicus, a bacterium associated with sugarcane. Current Science, v.76, p.874-875, 1999. MANULIS, S.; HAVIV-CHESNER, A.; BRANDL, M.T.; LINDOW, S.E.; BARASH, I. Differential involvement of indole-3-acetic acid biosynthetic pathways in pathogenicity and epiphytic fitness of Erwinia herbicola pv. gypsophilae. Molecular Plant?Microbe Interaction, v. 11, p. 634?642, 1998. MARSCHNER, H. Root-induced changes in the availability of mi c ronut r ient s in the rhi zosphere. In: WAISEL, Y.; ESHEL, A. e KAFKAFI, V. (Eds.). Plants roots: The hidden half. New York, Marcel Dekkerp, p.503-528, 1991. MARTIN, H.V.; ELLIOTT, M.C.; Ontogenetic Changes in the Transport of Indol-3yl-acetic Acid into Maize Roots from the Shoot and Caryopsis. Plant Physiology, v.74, n.4, p.971-974, 1984. McCULLY, M. Niches for bacterial endophytes in crop plants: a plant biologist?s view. Aust. Journal of Plant Physiology. v.28, p.983?990, 2001. MENNA, P.; HUNGRIA, M.; BARCELLOS, FG.; BANGEL, E.V.; HESS, PN.; MARTINEZ-ROMERO, E. Molecular phylogeny based on the 16S rRNA gene of elite rhizobial strains used in Brazilian commercial inoculants. SYSTEMATIC AND APPLIED MICROBIOLOGY, v.29, p.315?33, 2006. MOREIRA, F.M.S., SIQUEIRA, J.O. Microbiologia e bioqu?mica do solo. 2a ed. UFLA, Lavras, Brasil. 2006, 729 p. MORRIS, R. O. Genes specifying auxin and cytokinin biosynthesis in prokaryotes. In Davies, P.J. (ed.), Plant Hormones: Physiology, Biochemistry and Molecular Biology, Kluwer Academic Publishers, p.318?339, 1995. MUTHUKUMARASAMY, R.; CLEENWERCK, I.; REVATHI, G.; VADIVELU, M.; JANSSENS, D.; HOSTE, B.; GUM, K.U.; PARK, K.D.; SON, C.Y.; SA, T.M.; CABALLERO-MELLADO, J. Natural association of Gluconacetobacter diazotrophicus and diazotrophic Acetobacter peroxydans with wetland rice. Systematic and Applied Microbiology, v.28, p.277?286, 2005. NAGASAWA, T.; MAUGER, J. e YAMADA, H. A novel nitrilase, arylacetonitrilase, of Alcaligenes faecalis JM3. Purification and characterization. European Journal of Biochemistry, v.194, p.765?772, 1990. NARULA, N.; LAKSHMINARAYANA, K; TAURO, P. Ammonia excretion by Azotobacter chroococcum. Biotechnolog Science. v. 23, p. 467?470, 1980. NAUTIYAL, C. An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiology Letters, v.170, p.265-270, 1999. 59 NOVAIS, R. F.; SMYTH, J. T.; NUNES, F. N. F?sforo. In: NOVAIS, R. F, VENEGAS, V. H. A.; BARROS, N.F.; FONTES, R.L.F; CANTARUTTI, R.B J?LIO.; NEVES, C.L. (Eds.). Fertilidade do Solo. 1 ed. Vi?osa: Sociedade Brasileira de Ci?ncia do Solo, p. 471-550, 2007. OLIVEIRA, E. Quantifica??o da fixa??o biol?gica de nitrog?nio em arroz (Oryza sativa L.) inundado. 1994. 135p. Disserta??o (Mestrado) - Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ. OSBORNE, C. A.; GALIC, M.; SANGWAN, P.; JANSSEN, P. H. PCRgenerated artefact from 16S rRNA gene-specific primers. FEMS Microbiology Letters, v.248, p.183?187, 2005. PARK, K.H.; LEE, O.M.; JUNG, H.I.; JEONG, J.H.; JEON, Y.D.; HWANG, D.Y.; LEE, C.Y.; SON, H.J. Rapid solubilization of insoluble phosphate by a novel environmental stress-tolerant Burkholderia vietnamiensis M6 isolated from ginseng rhizospheric soil. Applied Microbiology and Biotechnology, v.86, p.947?95, 2010. PATTEN, C.L; GLICK, B.R. Regulation of indoleacetic acid production in Pseudomonas putida GR12-2 by tryptophan and the stationary phase sigma factor RpoS. Canadian journal microbiology, v,48, p.635-642, 2002a. PATTEN, C.L.; GLICK, B.R. The role of bacterial indoleacetic acid in the development of the host plant root system. Applied and Environmental Microbiology, v.68, p.3795-3801, 2002b. PATTEN, C. L.; GLICK, B. R. Role of Pseudomonas putida indole acetic acid in development of the host plant root system. Applied and Environmental Microbiology, v.68, p.3795-3801, 2002c. PEDRAZA, R.O.; BELLONE, C.H.; DE BELLONE, S.; BOA SORTE, P.M.B.; TEIXEIRA, K.R.D. Azospirillum inoculation and nitrogen fertilization effect on grain yield and on the diversity of endophytic bacteria in the phyllosphere of rice rainfed crop. European Journal of Soil Biology, v.45, p.36-43, 2009. PERIN, L. Estudo da Comunidade de Bact?rias Diazotr?ficas do G?nero Burkholderia em Associa??o com Cana-de-a??car e Descri??o de Burkholderia silvatlantica. 2007. 120 p. Tese (Doutorado em Agronomia - Ci?ncia do Solo) - Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ. PERLEY, J.W.; STOWE, B.B. On the ability of Taphrina deformans to produce indoleacetic acid from tryptophan by way of tryptamine. Plant Physiology, v.41, p.234?237, 1966. PII, Y.; CRIMI, M.; CREMONESE, G.; SPENA, A.; PANDOLfiNI, T. Auxin and nitric oxide control indeterminate nodule formation. BMC Plant Biology, v.7, p.1?11, 2007. POLY, F.; MONROZIER, L. J.; BALLY, R. Improvement in the rflp procedure for studying the diversity of nifH genes in communities of nitrogen fixers in soil. Research in Microbiology, v.152, p.95-103, 2001. 60 PUNSCHKE, K.; CARLOMAGNO, M.; LABANDERA, C. Potencial agron?mico de bacterias fijadoras de nitr?geno end?fitas de arroz. In: V Simposio de Recursos Geneticos para Am?rica Latina e el Caribe: Actas Uruguay, 2005. RADWAN, T.E.E.; MOHAMED, Z.K.; REIS, V.M. Efeito da inocula??o de Azospirillum e Herbaspirillum na produ??o de compostos ind?licos em pl?ntulas de trigo e arroz. Pesquisa Agropecuaria Brasileira, v.39, p.987?99, 2004. RAJKUMAR, M.; LEE, K.J.; LEE, W.H.; BANU, J.R. Growth of Brassica juncea under chromium stress: Influence of siderophores and indole 3 acetic acid producing rhizosphere bacteria. Journal of Environmental Biology, v.26, n.4, p.693?699, 2005. RAMAMOORTHY, V.; VIWANATHAN, R.; RAGUCHANDER, T.; PRAKASAM, V.; SAMIYAPPAN, R. Induction of systems resistance by plant growth promotion rhizobacteria in crop plants against pests and diseases. Crop Protection, v.20, p.1-11, 2001. RICHARDSON, A.E. Prospects for using soil microorganisms to improve the acquisition of phosphorus by plants. Australian Journal Plant Physiology, v.28, p.897?906, 2001. RICHARDSON, A. E. Making microorganims mobilize soil phosphorus. In: VAL?ZQUEZ, E.; RODR?GUEZ-BARRUECO, C. (Eds.). First international meeting on microbial phosphate solubilization, Dordrecht: Developments in Plant and Soil Science, v.102, p.85?90, 2007. ROBINSON, J .S.; SYERS, J .K. A critical evaluation of the factors influencing the dissolution of Gafsa phosphate rock. Journal of soil science, v.41, p.597-605, 1990. RODRIGUES, L. S. Estudo da diversidade de bact?rias diazotr? |
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