During spinal cord development, precursor cells transition from proliferative divisions to differentiative divisions. Traditionally proliferative divisions, which increase cell numbers, are thought to be symmetric, whereas differentiative divisions are thought to occur both by symmetric and asymmetric divisions. Currently, the mechanisms that control this differentiative cell division fate remain to be defined. However, studies conducted using atypical protein kinase C (aPKC) suggests that aPKC has a conserved function in controlling cell division orientation. In this study, we look at the role aPKC may play in maintaining precursor division in the zebrafish spinal cord. Through time-lapse imaging and loss of function studies we were able to show that aPKC does regulate precursor division in the zebrafish spinal cord, and in its absence excess oligodendrocyte precursor cells (OPCs) are specified at the possible expense of adult precursors.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-07132009-114737 |
| Date | 19 July 2009 |
| Creators | Roberts, Randolph K. |
| Contributors | Chris Janetopoulos, E. Michelle Southard-Smith, Joshua Gamse, Bruce Appel, Lilianna Solnica-Krezel |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-07132009-114737/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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