Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany Candidate: Mgr. Eliška Kohelová Supervisor: prof. Ing. Lucie Cahlíková, Ph.D. Title of Doctoral Thesis: Alkaloids of Zephyranthes citrina (Amaryllidaceae): isolation, structure identification, biological activity. Key words: Amaryllidaceae alkaloids, Zephyranthes, Alzheimer's disease, AChE, BuChE, POP, oncological diseases Zephyranthes citrina Baker was chosen, based on result of previous screening study and literatury research, for detailed phytochemical work for the purpose of isolation of the widest range of AAs. From 35 kg of fresh bulbs was obtained 151 g of purified alkaloidal extract, which was processed using column chromatography to almost 700 fractions. These fractions were connected into 27 subfractions, which were processed by liquid-liquid extraction, flash chromatography, preparative TLC and crystallization. Finally, 27 pure alkaloids were isolated. All compounds were identified by MS (EI, ESI), HRMS, 1D- and 2D-NMR, CD, optical rotation and by comparison with literature data. Seven compounds were identified as new undescribed alkaloids (6α-ethoxyhippeastidine, 10-deoxy-6α-ethoxyhippeastidine, narcieliine, zephyjanine, zephycitrine I, 7-ethoxy-10-methoxy-1-methyllycorenane-9-ol a zephycitrin...
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:451702 |
| Date | January 2021 |
| Creators | Kohelová, Eliška |
| Contributors | Cahlíková, Lucie, Lapčík, Oldřich, Mučaji, Pavel |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/doctoralThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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