Thesis (S.M. in Toxicology)--Massachusetts Institute of Technology, Biological Engineering Division, 2004. / Includes bibliographical references (leaves 45-47). / Design and fabrication of a microfluidics system capable of generating reproducible and controlled micro-biochemical environments that can be used as a diagnostic assay and microreactor is important. Here, a simple technique was developed to create a robust microfluidics system capable of generating precise gradients of biochemical properties within its channels. Through this approach, it is possible to create a gradient generator with mammalian cells patterned and seeded under its poly(dimethylsiloxane) (PDMS) channels. Cells that were seeded and patterned under the PDMS channels remained viable and capable of performing intracellular reactions. Using the gradient generator within the PDMS microfluidic device, a gradient of specific and controlled biochemicals can be flowed on seeded cells allowing for high-throughput molecular interaction analysis. The microfluidics system provides a way to study and analyze cell response in the presence of a combination of biochemical signals. / by Guan-Jong Chen. / S.M.in Toxicology
| Identifer | oai:union.ndltd.org:MIT/oai:dspace.mit.edu:1721.1/18043 |
| Date | January 2004 |
| Creators | Chen, Guan-Jong, 1981- |
| Contributors | Robert S. Langer., Massachusetts Institute of Technology. Biological Engineering Division., Massachusetts Institute of Technology. Biological Engineering Division. |
| Publisher | Massachusetts Institute of Technology |
| Source Sets | M.I.T. Theses and Dissertation |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Format | 47 leaves, 2882891 bytes, 2886580 bytes, application/pdf, application/pdf, application/pdf |
| Rights | MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission., http://dspace.mit.edu/handle/1721.1/7582 |
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