Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biological Engineering, 2015. / Cataloged from PDF version of thesis. / Includes bibliographical references. / Glioblastoma (GBM) is the most common malignant form of brain cancer. Even with treatment including surgery, radiation, and temozolomide chemotherapy, the 1 year survival rate is only 35%. To identify specific mediators of GBM progression in a genetically engineered murine model of proneural GBM, we quantified signaling networks using mass spectrometry. We identified oncogenic signaling associated with the GBM model, such as increased phosphorylation of ERK1/2, P13K, and PDGFRA, relative to murine brain. Phosphorylation of CDK₁ Y₁₅, which causes G₂ /M cell cycle arrest, was measured to be the most differentially phosphorylated site, with a 14-fold increase in the tumors. We used syngeneic cell lines to investigate this checkpoint further and treated these cells with MK-₁₇₇₅, an inhibitor of Wee₁, the kinase responsible for phosphorylation of CDK₁ Y₁₅. MK-₁₇₇₅ treatment resulted in mitotic catastrophe of these cells, as measured by increased DNA damage, abnormal percentages of cells in cell cycle phases, and death by apoptosis. This response was abrogated by inhibiting CDK₁ with roscovitine, a CDK inhibitor, demonstrating the necessity of active CDK₁ for MK-₁₇₇₅ induced mitotic catastrophe. To assess the extensibility of targeting Wee₁ and the G₂/M checkpoint in GBM, we treated patientderived xenograft (PDX) cell lines with MK-₁₇₇₅. The response was more heterogeneous, but we measured decreased CDK₁ phosphorylation, increased DNA damage, and death by apoptosis. These results were validated in a flank GBM PDX model where treatment with MK-₁₇₇₅ increased mouse survival by 1.74-fold. We also quantified the signaling differences in our murine GBM model after treatment with sunitinib, an inhibitor of its driver receptor tyrosine kinase, PDGFRA. Treatment increased survival but lead to a morphological change causing a more invasive phenotype. Pro-migratory signaling was characterized by mass spectrometry, such as increased phosphorylation of Eno₁, ELMO₂, and tubulins. Invasion was further characterized in a lung cancer model where we identified signaling specific to different ligands that result in similar levels of invasion. We have demonstrated that unbiased, quantitative phosphotyrosine proteomics has the ability to reveal therapeutic targets in tumor models and signaling differences between treatments. / by Rebecca S. Lescarbeau. / Ph. D.
| Identifer | oai:union.ndltd.org:MIT/oai:dspace.mit.edu:1721.1/99055 |
| Date | January 2015 |
| Creators | Lescarbeau, Rebecca S. (Rebecca Susan) |
| Contributors | Forest M. White., Massachusetts Institute of Technology. Department of Biological Engineering., Massachusetts Institute of Technology. Department of Biological Engineering. |
| Publisher | Massachusetts Institute of Technology |
| Source Sets | M.I.T. Theses and Dissertation |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Format | 187 pages, application/pdf |
| Rights | MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission., http://dspace.mit.edu/handle/1721.1/7582 |
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